Dental caries is an infectious disease that is caused by the interaction between the host, the diet, and a diverse microbial community. This study illustrated the anticariogenic effects of THYJ-15 against Streptococcus mutans, a major agent of dental caries. THYJ-15 was identified as Latilactobacillus sakei subsp. sakei through 16S rRNA sequencing, with a similarity of 99.72% to L. sakei subsp. sakei JCM 1157T. The cell-free supernatant of L. sakei subsp. sakei THYJ-15 observed MIC values of 10 mg/mL and MBC values of 20 mg/mL against S. mutans KACC 16833T. The cell-free supernatant of L. sakei subsp. sakei THYJ-15 showed significant inhibitory effects on biofilm formation, acid production and glucosyltransferases(GTFs) production against S. mutans. As a results, it was confirmed that L. sakei subsp. sakei THYJ-15 is a lactic acid bacterium possessing notable anticariogenic effects. Accordingly, L. sakei subsp. sakei THYJ-15 is expected to have great potential to be used as a basic substance for dental caries treatment caused by S. mutans.

서 론
재료 및 방법
    사용 균주
    김치 유래 유산균의 분리 및 동정
    항균 활성 확인
    생물막 형성 억제 효과
    유기산 억제 효과
    Glucosyltransferase(GTFs) 생성 억제 효과
    통계분석
결과 및 고찰
    김치 유래 유산균 THYJ-15의 분리 및 동정
    THYJ-15 배양액의 Streptococcus mutans에 대한 항균 효과
    THYJ-15 배양액의 생물막 형성 억제 효과
    THYJ-15 배양액의 유기산 생성 억제 효과
    THYJ-15 배양액의 Glucosyltransferase(GTFs) 억제 효과
요약

• 정제용(경희대학교 일반대학원 생명공학원) | Je-Yong Jung (Department of Biotechnology, Kyunghee University, Gyeonggi 17104, Republic of Korea)
• 유두나(경희대학교 일반대학원 생명공학원) | Du-Na Yu (Department of Biotechnology, Kyunghee University, Gyeonggi 17104, Republic of Korea)
• Nguyen Thi Minh Trang(경희대학교 일반대학원 생명공학원)
• 이태후(경희대학교 일반대학원 생명공학원) | Tae-Hoo Yi (Department of Biotechnology, Kyunghee University, Gyeonggi 17104, Republic of Korea) Corresponding author
• 권예지(경희대학교 생명과학대학 한방생명공학과) | Ye-Ji Kwon (Department of Oriental Medicinal Biotechnology, Kyunghee University, Gyeonggi 17104, Republic of Korea)
• 이은지(경기대학교 대학원 대체의학과) | Eun-Ji Yi (Department of Alternative Medicine, Kyonggi University, Seoul 03746, Republic of Korea)
• 국무창(배화여자대학교 식품영양학과) | Moochang Kook (Department of Food & Nutrition, Baewha Women's University, Seoul 03039, Republic of Korea)