논문 상세보기
pp.600-605
젖산균의 생육은 10㎍/ml의 알루미늄이 첨가된 broth system에서 정상적으로, Streptococcus속보다는 Lactobacillus속이 더 높은 내성을 가진 것으로 나타냈다. 알루미늄 50㎍/ml이 첨가된 배지에서 24시간 배양시킨 젖산균에 축적된 알루미늄 양은 3.52∼4.93㎍/g wet cell로 3.08㎎의 Clostridium perfringens ATCC 3627 보다 높았으며 젖산균 중 Lactobacillus bulgalicus ATCC 11842의 축적량이 가장 높았다. Lactobacillus acidophilus ATCC 4356 균체내의 알루미늄 분포상태는 세포벽에 49.1%, 원형질막에 27.3%, 세포질 23.6%로 나타났다.
Present study was investigated to evaluate the aluminum absorption effect on lactic acid bacteria(Lactobacillus acidophilus ATTC 4356, Lactobacillus bulgaricus ATTC 11842, Lactobacillus casei IFO 3533, and Streptococcus thermophilus KCTC 2185 ; LAB) and Clostridium perfringens ATCC 3627(CP) in artificial intestinal tract. Their growth rate, aluminum accumulation and cellular distribution was studied under anaerobic broth system. All of above microbes were inhibited by adding 10 to 100 ppm of aluminum. The degree of aluminum in LAB(Lactobacillus acidophilus ATCC 4356, Lactobacillus bulgaricus ATCC 11842, Lactobacillus casei IFO 3533, and Streptococcus thermophilus KCTC 2185) was higher than of CP. The largest amount of aluminum was accumulated in Lactobacillus bulgaricus ATCC 11842. Aluminum accumulation in LAB was distributed in 49.1% at cell wall, 27.3% at plasma membrane, and 23.6% at cytoplasm, respectively. This study suggests that LAB might help to eliminate the ingested aluminum in intestinal tract.
