Peanut is a well-known food allergen that causes adverse reactions ranging from mild urticaria to life-threatening anaphylaxis. Consumers suffering from peanut allergies should thus avoid consuming undeclared peanuts in processed foods. Therefore, effective cleaning methods are needed to remove food allergens from manufacturing facilities. To address this, wet cleaning methods with washing water at different temperatures, abstergents (peracetic acid, sodium bicarbonate, dilute sodium hypochlorite, detergent), and cleaning tools (brush, sponge, paper towel, and cotton) were investigated to remove peanuts from materials used in food manufacture, including plastics, wood, glass, and stainless steel. Peanut butter was coated on the surface of the glass, wood, stainless steel, and plastic for 30 min and cleaned using wet cleaning. The peanut residue on the cleaned surfaces was swabbed and determined using an optimized enzyme-linked immunosorbent assay (ELISA). Cleaning using a brush and hot water above 50oC showed an effective reduction of peanut residue from the surface. However, removing peanuts from wooden surfaces was complicated. These results provide information for selecting appropriate materials in food manufacturing facilities and cleaning methods to remove food allergens. Additionally, the cleaning methods developed in this study can be applied to further research on removing other food allergens.

ABSTRACT
Materials and Methods
    시료 수집 및 땅콩 알레르겐 처리
    간접효소면역분석법(indirect enzyme-linked immunosorbentassay; iELISA)
    식품 제조시설 조리도구 표면에 대한 식품알레르기 세척법 확립
Results and Discussion
    땅콩 검출을 위한 iELISA법의 특성
    불림 및 세척용수 온도와 세척용제의 사용에 따른 땅콩 알레르겐 제거효과 확인
    땅콩 알레르겐의 효율적인 세척을 위한 세척도구 선정
    세척용액을 이용한 세척 효과 확인
Acknowledgement
국문요약
Conflict of interests
ORCID
References

• 김솔아(경상국립대학교 대학원 응용생명과학부) | Sol-A Kim (Division of Applied Life Science, Graduate School, Gyeongsang National University, Jinju, Korea)
• 이정은(경상국립대학교 스마트팜 연구센터) | Jeong-Eun Lee (Institute of Smart Farm, Gyeongsang National University, Jinju, Gyeongnam, Korea)
• 신재민(전남대학교 치의학전문대학원 학석통합과정) | Jaemin Shin (Department of Dentistry, Chonnam National University, Gwangju, Korea)
• 심원보(경상국립대학교 스마트팜 연구센터, 경상국립대학교 식품공학과, 경상국립대학교 농업생명과학연구원) | Won-Bo Shim (Institute of Smart Farm, Gyeongsang National University, Division of Food Science and Technology, Gyeongsang National University, Institute of Agriculture and Life Science, Gyeongsang National University) Corresponding author