Lyophyllum decastes has been used for culinary purpose. The present study was conducted to evaluate the antioxidant and anti-inflammatory effects from methanol, acetone, and hot water extracts of L. decastes fruiting bodies. The acetone and methanol extracts showed the higher 1,1-diphenyl-2-picryl-hydrazy radical scavenging activities than that of the hot water extract at 0.5–2.0 mg/mL and was comparable to the BHT, the positive control. The ferrous ion chelating effects of the mushroom extracts at 0.5–2.0 mg/mL were significantly higher than that of BHT. The reducing power of acetone extract (2.12) was significantly lower than that of BHT (2.73) at 2.0 mg/ mL. The mushroom extracts also showed inhibitory effects on production of nitric oxide (NO), and expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide-induced murine macrophage cells in a concentration dependent manner. In vivo anti-inflammatory experiment on carrageenan-induced hind-paw edema of rat model, the acetone extract of the mushroom significantly suppressed the carrageenan-induced rat hind paw edema of rats in a dose dependently. The results suggest that the fruiting bodies of Lyophyllum decastes are a good natural resource of antioxidant and anti-inflammation.

INTRODUCTION
MATERIALS AND METHODS
    Mushrooms and extraction
    Animals
    Assay for the total phenol content
    Assay for the total flavonoid content
    Evaluation of antioxidant activity
    Reducing Power
    Cell viability assay
    Evaluation of anti-inflammatory activity
    Western blot analysis
    Carrageenan-Induced hind paw edema in rats
    Statistical analysis
RESULTS AND DISCUSSION
    Evaluation of phenolic contents
    Evaluation of flavonoid contents
    Evaluation of Antioxidant Activities
    Chelating effect on ferrous ions
    Assay for cytotoxicity
    Anti-inflammatory activity of fruiting body extractsof L. decastes
    Western Blot analysis
    Carrageenan-induced paw edema in rats

• Ki Nam Yoon(Department of Clinical Laboratory Science, Ansan University, 155 Ansan Dae-hak-ro, Sangrok-gu, Ansan 15328, Korea)
• Tae Soo Lee(Division of Life Sciences, Incheon National University, (Songdo-dong) 119 Academy-ro, Yeonsu-gu, Incheon 22012, Korea) Corresponding author