In this study, the protein content and functional changes in soybeans cultured with Phellinus linteus HN00K9 were analyzed. P. linteus HN00K9 was cultured on soybeans. The crude protein content in soybeans cultured with HN00K9 (PMS) was 41.99%, which was higher than that in soybeans not cultured with the mushroom (UCS). The total free amino acid content in PMS increased to 39,963 mg/100 g, which was higher than that in UCS (36,817 mg/100 g). In particular, in PMS, glutamic acid accounted for 18.5% of the total amino acids at 7,413 mg/100 g. The total polyphenol content in PMS was 2.66 mg GAE/g, which was more than 45% higher than the amount in UCS (1.45 mg GAE/g). Additionally, PMS showed a DPPH radical scavenging activity of 33.3%, which was 3 times higher than that exhibited by UCS (11.5%), reflecting its high antioxidant content. Therefore, the PMS in this study has potential for use as a functional food material.

서 론
재료 및 방법
    목질진흙상황버섯대두 균사체 배양
    아미노산분석
    단백질함량
    총 폴리페놀 함량
    2,2-diphenyl-1-picrylhydrazyl (DPPH) 라디칼 소거능
    통계처리
결과 및 고찰
    목질진흙버섯 HN00K9의 대두 균사체 배양
    목질진흙버섯 HN00K9 균사체 배양에 의한 대두의 단백질 및 아미노산 함량변화
    항산화 활성
적 요
감사의 글

• 김자윤(한경국립대학교 생명공학부) | Ja-Yoon Kim (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea)
• 백예린(한경국립대학교 생명공학부) | Ye-Lin Baek (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea)
• 이상호(㈜ 마이띵스) | Sang-Ho Lee (Mythings Co., Ltd., Daejon 34139, Korea)
• 강희완(한경국립대학교 생명공학부, 한경국립대학교 유전공학연구소) | Hee-Wan Kang (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea) Corresponding author