The objective of this study was to achieve biological control of green mold disease in Pyogo mushrooms using antagonistic microorganisms. Bacillus subtilis BSM320 cells inhibited mycelial growth by 48–60% against three Trichodermaisolates including T. hazianumisolated from the substrates of Lentinula edodes, showing their antifungal activity.The bacteria were cultured to a high density of 4.2 x 109±113.7 cfu/mlin aqueous extract of composted spent mushroom substrates of L. edodes containing 1% glucose and showed a higher growth rate than that observed when using the commercial medium, Luria-Bertani broth. The bacterial culture showed a 75% protective effect without damaging the mushroom fruiting bodies. These results suggest that B. subtilis BSM320culture is suitable for biological control of green mold disease during mushroom cultivation.

서 론
재료 및 방법
    표고수확후배지 퇴비 추출 배지 제조 및 B. subtilisBSM320 배양
    항균활성검정
    표고 푸른곰팡이병 발병도
    통계처리
결과 및 고찰
    B. subtilis BSM320의 푸른곰팡이균 Trichoderma spp.에 대한 항균 할성
    LeCSMS 물 추출물을 이용한 B. subtilis BSM320의 고농도 배양
    B. subtilis BSM 320 배양액을 이용한 표고 푸른곰팡이병의 생물학적 방제
적 요
감사의 글

• 김자윤(한경국립대학교 생명공학부) | Ja-Yoon Kim (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea.)
• 박세현(한경국립대학교 생명공학부) | Se-Hyun Park (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea.)
• 박성준(한경국립대학교 생명공학부) | Seong-Joon Park (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea.)
• 황보주형(한경국립대학교 생명공학부) | Ju-Hyeong Hwang Bo (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea.)
• 강희완(한경국립대학교 생명공학부, 한경국립대학교 유전공학연구소) | Hee-Wan Kang (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea) Corresponding author