In general, the design response spectrum in seismic design codes is based on the mean-plus-one-standard deviation response spectrum to secure high safety. In this study, response spectrum analysis was performed using seismic wave records adopted in domestic horizontal design spectrum development studies, while three response spectra were calculated by combining the mean and standard deviation of the spectra. Seismic wave spectral matching generated seismic wave sets matching each response spectrum. Then, seismic fragility was performed by setting three damage levels using a single-degree-of-freedom system. A correlation analysis was performed using a comparative analysis of the change in the response spectrum and the seismic fragility concerning the three response spectra. Finally, in the case of the response spectrum considering the mean and standard deviation, like the design response spectrum, the earthquake load was relatively high, indicating that conservative design or high safety can be secured.