Abstract Purpose : This study investigated the impact of knowledge and awareness of perception of harmful rays on the use of blue light blocking lenses among university students majoring in optometry. Methods : This study targeted 65 optometry students who responded to an online survey from September 2023 to October 2023. The survey used a survey tool with a total of 15 questions, including knowledge and awareness of perception of harmful rays and demands for guidelines. Statistical analysis was performed using SPSS 19.0. Results : The overall knowledge score about UV rays and blue light was 14.00±3.10 for optometry students and 12.30±3.50 for general university students, showing that the optometry students had higher knowledge (p<0.001). In the case of use of UV-blocking lenses, 70.8% of optometry students were found to be using them, which was higher than 51.5% of general university students. The use of glasses to block blue light was found to be 41.5% of optometry students and 36.9% of general university students. The correlation between perception of the importance of blocking harmful rays and the need for guidelines for the correct selection of blue light blocking lenses was r=0.528 (p<0.001). Conclusion : Optometry students' knowledge and perception of harmful rays were found to be higher than those of general university students, and it appears to have an impact on the use of harmful ray-blocking lenses. Therefore, it is necessary to strengthen education and establish guidelines for the correct selection of harmful ray blocking lenses.