Abstract Purpose : The purpose of this study is to evaluate the differences and reliability of various methods for measuring Interpupillary Distance. Methods : The participants were 50 Cambodian adults (28.08±3.85 years old) without ocular disease and abnormal binocular vision. Far/near IPD was measured using Corneal reflection pupillometer, PD ruller and spotting, and calculated formula for fixation distance. Using each method, the pupil distance of both eyes was measured three times, respectively, and the average value was recorded. Results : The average value of the corneal reflection pupilometer in the distance was 63.08±3.42 mm, the average value of PD calculated formula was 62.97±3.41 mm, the average value of the PD ruler was 63.72±3.17 mm, and the average value of the spotting method was 63.89±3.15 mm. The average value of the corneal reflection pupilometer in the near was 59.85±3.23 mm, the average value of the calculated formula for fixation distance was 59.95±3.23 mm, the average value of PD ruler was 59.72±3.29 mm, and the average value spotting method was 59.47±3.23 mm. Comparison between various methods in the distance and near showed statistically significant differences (p<0.001). Conclusion : In the comparative analysis between each method, the corneal reflection pupillometer is considered to be the most accurate method, but since there is no significant difference from the average value of the Spotting method, the PD Ruler Viktorin method, and the calculated formula for fixation distance, whichever method is used, it is clinically acceptable. Therefore it is believed that each method is compatible with each other. Key words : Corneal reflection pupillometer, PD meter, PD ruler, Spotting