Herbicide-resistant transgenic rapeseed (TG rapeseed) was developed by inserting phosphinothricin acetyltransferase (PAT, bar), a modified gene from the soil bacterium Streptomyces hygroscopicus, into the genome of a conventional variety of rapeseed (Youngsan). The TG rapeseed used for the test was confirmed to express the PAT gene by polymerase chain reaction (PCR) and immunostrip. Feeding tests were conducted with Cyprinus carpio to evaluate the environmental risk of TG rapeseed, including the herbicide resistant gene. C. carpio was fed 100% ground rape suspension, TG rapeseed, or non-genetically modified (GM) counterpart rapeseed (Youngsan). As a result, the feeding test showed no significant differences in the cumulative immobility or abnormal response between C. carpio samples fed on TG rapeseed and non-GM counterpart rapeseed. The 48 h-LC50 values of the TG rapeseed and the non-GM counterpart rapeseed were 3,376 mg/L (95 % confidence limits: 3,169 - 3,596 mg/L) and 2,682 mg/L (95 % confidence limits: 2,267 – 3,123 mg/L), respectively. The rape NOEC (no observed effect concentration) value for C. carpio was suggested to be 625 mg/L. Based on these results, there was no significant difference in the toxicity for non-target organisms (C. carpio) between the TG rapeseed and non- GM counterparts.

서 언
재료 및 방법
실험 재료
    게놈 DNA 추출 및 polymerase chain reaction (PCR)검정
    제초제 저항성 GM유채의 목적 단백질 발현 분석
    잉어(C. carpio) 배양 조건
    잉어에 대한 시료 처리조건
    급성 독성 분석을 위한 조사 항목
결과 및 고찰
    제초제저항성 GM유채의 분자생물학적 분석
    시료처리용 시험용수의 수질변화 검정
    영산유채와 제초제 저항성 GM유채의 처리 농도별 잉어치사 개체 분석
    영산유채와 제초제 저항성 GM유채의 급이에 의한 잉어에대한 급성독성
적 요
ACKNOWLEDGMENTS
REFERENCES

• 오성덕(농촌진흥청 국립농업과학원 생명자원부) | Sung-Dug Oh (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea)
• 백경령(농촌진흥청 국립농업과학원 생명자원부) | Kyunglyung Baek (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea)
• 민석기(한국화학융합시험연구원) | Seok-Ki Min (Korea Testing & Research Institute, Hwasun 58141, Korea)
• 홍준기(농촌진흥청 국립농업과학원 생명자원부) | Joon Ki Hong (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea)
• 윤도원(농촌진흥청 국립농업과학원 생명자원부) | Doh-Won Yun (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea)
• 이성곤(농촌진흥청 국립농업과학원 생명자원부) | Seong-Kon Lee (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea)
• 장안철(농촌진흥청 국립농업과학원 생명자원부) | Ancheol Chang (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea) Corresponding author