With the increase of temporarily-stored radioactive waste in Korea, the disposal of radioactive waste in a deep geological repository, which is located in crystalline rock at a depth of hundreds of meters below the ground level, has received great attention nowadays. To ensure the permanent isolation of radionuclides from the human and surrounding ecosystems, the safety assessment for the high-level radioactive waste disposal facilities is essential. For the reliable safety assessment of fractured rock, it is especially important to input proper hydraulic properties of fractures such as aperture and hydraulic conductivity, which can directly affect the fluid flow and radionuclide transport. Meanwhile, it has become important to consider sudden fault behavior caused by an earthquake with the recent occurrence of high-intensity earthquakes in the Korean Peninsula. The sudden fault behavior can induce the changes of the hydraulic properties of fractures. Since the changes of the hydraulic properties directly affects to the radionuclide transport in the fractured rock, it is important to estimate the effect of earthquake-induced stress change on hydraulic properties of fractures in the perspective of long-term safety assessment. In this study, the effect of an earthquake on the hydraulic properties of fractures was explored by a numerical approach. The static Coulomb stress change after the earthquake was calculated using software ‘Coulomb 3’ developed by United States Geological Survey (USGS) with the assumption for several mechanical properties such as Young’s modulus, Poisson’s ratio and effective coefficient of friction. The final stress after earthquake occurrence was calculated as the sum of the initial stress and the stress change. Thereafter, the normalized transmissivity of fracture after the earthquake was calculated using the final stress from the stress-transmissivity relationship. Using the methodology for calculating fracture transmissivity change induced by the earthquake developed in this study, the effect of several factors, such as the earthquake magnitude and the distance between fracture and epicenter, was additionally explored. The newly developed methodology will be applied to the processbased total system performance assessment framework (APro) being developed by KAERI, and this study is expected to be helpful for the safety assessment considering long-term evolution phenomena including earthquakes.