In this study, we evaluated fracture filling minerals and aperture distribution along the fracture surfaces under the controlled conditions. The fractured granite block which has a single natural fracture of 1 m scale was sampled in a domestic quarry (Iksan), which groundwater had been flowed through. This rock has an interconnected porosity of 0.36 with the specific gravity of 2.57. The experimental setup with the granite block with dimensions of 100×60×60 (cm). The fracture is sealed with rock silicone rubbers when it intersects the outer surfaces of the block and the outer surfaces are coated with the silicone to prevent loss of water by evaporation. Nine boreholes were drilled of orthogonal direction at the fracture surface. A flow of de-ionized water through the fracture between pairs of boreholes was initiated and the pressure required to maintain a steady flow was measured. In additions, fracture filling minerals were sampled and examined by mineralogical and chemical analyses. There are phyllosilicate minerals such as illite, kaolinite, and chlorite including calcite, which are fracture filling minerals. The illite and kaolinite usually coexist in the fracture, where their content ratio is different according to which mineral is predominant. For the evaluation of fracture, surface was divided into an imaginary matrix of 20×20 sub-squares as schematically. The calculated results are expressed as a two dimensional contour and a three dimensional surface plot for the aperture distribution in the fracture. The aperture value is distributed between 0.075 and 0.114 mm and the mean aperture value is 0.082 mm. The fracture volume is about 49 ml. These results will be very useful for the evaluation of environmental factor affecting the nuclides migration and retardation.