Background: Brassica oleracea var. italica (broccoli), a rich source of antioxidants,
can prevent various diseases and improve human health. In this study, we investigated
the antioxidative effects of broccoli sprout extract on oxidative stress induced by
lipopolysaccharide and cisplatin in cell and organ tissue models.
Methods: Antioxidative effect of BSE was evaluated using DPPH and ABTS in RAW
364.7 cells, and effects of BSE on testes were investigated using Cisplatin-induced
testicular damage model with an in vitro organ culture system.
Results: The DPPH assay showed that the antioxidant activity of the alcoholic
broccoli sprout extract was higher than that of the water extract. Additionally, the
expression levels of antioxidation-related genes, Nrf2 , Gsr , HO-1, and catalase , were
significantly increased in broccoli sprout extract-treated RAW 264.7 cells, and the
extract suppressed lipopolysaccharide-induced mitochondrial dysfunction. Based on
the results in the RAW 264.7 cell culture, the antioxidative effects of the extracts were
investigated in a mouse testis fragment culture. The expression of Nrf2 , HO-1 , and
Ddx4 was clearly decreased in cisplatin-treated mouse testis fragments and not in
both broccoli sprout extract- and cisplatin-treated mouse testis fragments. In addition,
the oxidative marker O-HdG was strongly detected in cisplatin-treated mouse testis
fragments, and these signals were reduced by broccoli sprout extract treatment.
Conclusions: The results of this study show that broccoli sprout extracts could serve
as potential nutraceutical agents as they possess antioxidant effects in the testes.

INTRODUCTION
MATERIALS AND METHODS
    Preparation of alcoholic BSE
    DPPH and ABTS assays
    Cell culture and treatment
    CellROX staining and flow cytometry
    Preparation and culture of mouse testis fragments(MTFs)
    Isolation of RNA and qPCR analysis
    Western blotting
    Histology and immunostaining
    Statistical analysis
RESULTS
    Antioxidant activity of BSE
    BSE increased the expression of antioxidation-relatedgenes in LPS-induced oxidative stress
    BSE inhibited mitochondrial dysfunction in cells withLPS-induced oxidative stress
    BSE suppressed the cisplatin-induced reduction inantioxidant activity in testicular tissue
DISCUSSION
CONCLUSION
REFERENCES

• Won-Young Lee(Department of Livestock, Korea National University of Agriculture and Fisheries, Jeonju 54874, Korea)
• Hyun-Woo Shim(Department of Animal Biotechnology, College of Life Science, Sangji University, Wonju 26339, Korea)
• Hyun-Jung Park(Department of Animal Biotechnology, College of Life Science, Sangji University, Wonju 26339, Korea) Corresponding author