Background: Largemouth bass (Micropterus salmoides) is introduced species that has caused aquatic ecology activity both in vitro and in vivo were investigated for the possibility of application of the bass extract as an alternative feed ingredient. Methods: The bass oil was extracted using a 1-L supercritical extractor, while the protein was extracted from 250 g of bass dry matter, which was dissolved in 1 mL of H2O at 50℃. Both oil and protein extracts were evaluated antioxidant activities and the level of DPPH radical scavenging assay and nitric oxide (NO) production assay with lipopolysaccharide response. Oral administration of 6.6 μL/g bass protein and 5.38 μL/g bass oil conducted for investigating serological and physiological effect. Results: DPPH radical scavenging showed similar radical scavenging ability of 50 μM of ascorbic acid at 200 μg of protein and 10% of oil treatment. NO concentration was diminished by the treatment of bass oil. Oral administration of both bass oil and proteins to mice showed that the body weight increase rate of the bass oil treated group was significantly reduced by 1.55 g compared to the other groups. The number of white blood cells (WBC) was increased by 4.52 k/μL in the bass protein-treated group and 4.44 k/μL in the bass oil-treated group compared to the control group. However, the serum IgG level did not show a significant difference between the bass extract-treated groups and the control group. Conclusions: These studies demonstrate that both bass oil and proteins extracted from the bass not only provide excellent effects of antioxidant and immune activity but can also be used as functional food supplements.

INTRODUCTION
MATERIALS AND METHODS
    Extraction and analysis of oil and protein fromMicropterus salmoides
    DPPH radical scavenging activity of oil and protein
    Nitric oxide assay of bass extraction and mixture
    Cytotoxic analysis of bass extraction in Madin-Darbycanine kidney cells
    Oral administration to mice and weight measurement
    Blood collection and analysis in mice
    Enzyme-linked immunosorbent assay forImmunoglobulin G analysis
    Statistical analysis
RESULTS
    Oil extraction and fatty acids analysis
    Soluble protein extract and taurine analysis
    Cytotoxic effects and antioxidant activities oflargemouth bass extracts
    Effects of largemouth bass extracts on physiologicalchanges in mice
    Complete blood count of mice treated with largemouthbass extracts
    IgG concentration in mice treated with largemouthbass extracts
DISCUSSION
REFERENCES

• Min-Gi Han(Department of Stem Cells and Regenerative Technology, Konkuk University, Seoul 05029, Korea)
• Ran Lee(Department of Stem Cells and Regenerative Technology, Konkuk University, Seoul 05029, Korea)
• Hyun Jung Park(Department of Animal Biotechnology, Sangji University, Wonju 26339, Korea)
• Kyung Hoon Lee(Research Institute, Bellys Co. Ltd, Incheon 22219, Korea)
• Hyuk Song(Department of Stem Cells and Regenerative Technology, Konkuk University, Seoul 05029, Korea) Corresponding author