In Korea, Kori Unit 1 and Wolsong Unit 1, have been permanently shut down in 2017 and 2019, and more nuclear power plants are expected to be permanently shut down after continued operation successively. Spent fuel has been generated during operation and stored in spent fuel pools. Due to the expected saturation of spent fuel pools within the next several decades, transportation of a huge amount of spent fuel is anticipated to interim storage facilities or final disposal facilities, even though the specific location is not decided. The U.S. Nuclear Regulatory Commission (NRC) states that every environmental report prepared for the licensing stage of a Pressurized Water Reactor shall contain a statement concerning risk during the transportation of fuel and radioactive wastes to and from the reactor. Thus, the licensee should ensure that the radiological effects in accidents, as well as normal conditions in transport, do not exceed certain criteria or be small if cannot be numerically quantified. Specific conditions that a full description and detailed analysis of the environmental effects of transportation of fuel and wastes to and from the reactor are exempted are specified in 10 CFR Part 51. Since there are no official requirements for radiological dose assessment for workers and public during the transportation of spent fuel in Korea, the margin when applying the U.S. regulatory criteria to the environmental impact assessment during the transport of spent fuel generated from domestic nuclear power plants is evaluated. A different approach would be needed due to the difference in the characteristics of spent fuel and geographical features.