Honeybees are pollinating agent of economic importance, and a model organism for microbiome analysis. The microbiota plays a great role in honeybee health and development as such needs to be fully elucidated among Apis mellifera in Korea. using 16S rRNA gene illumina sequencing, the gut microbiota of Apis mellifera at four developmental stages under natural condition shows dysbiosis of essential microbiota, especially between the 5-days larvae and workers.