The aim of this study was to investigate antibacterial activities of essential oil from C. morifolium against four Gram-positive bacteria and six Gram-negative bacteria. The antibacterial activity of the oils was determined by agar-well diffusion assay, minimum inhibitory concentration(MIC), and minimum bactericidal concentration(MBC). Essential oil of C. morifolium had a large inhibition zones especially against Salmonella enterica(21 mm) and Bacillus cereus(l9 mm). Essential oil of C. morifolium generally show4 higher antibacterial activity against Gm-positive bacteria than Gram-negative bacteria. MIC of essential oil from C. morifolium was 5㎕/㎖ against ten food-borne pathogens. MBC values were determined to be from 5 to 20㎕㎖ against eight bacteria except Salmonella choleraesuis and Listeria monocytogenes. Therefore, the essential oil of C. morifolium and its components have a potent antibacterial activity against food-borne pathogens, and is expected to be used as a novel food preservative.