Background: Self-myofascial release using a foam roller has short-term effects for improving muscle function and joint range of motion (ROM) and reducing delayed onset muscle soreness (DOMS) after exercise. Objectives: The purpose of our study was to examine the muscle physiological changes for each set in self-myofascial release of the hamstring muscles using a foam roller in order to provide basic data for the most effective program composition for improving muscle tension, stiffness, and flexibility of the hamstring muscles. Design: A quasi-experimental clinical trial. Methods: To confirm the effect of self-myofascial release of the hamstring muscles using a foam roller, muscle tone and stiffness of the hamstring muscles were measured. As an intervention method, the study subject performed four sessions of self-myofascial release by moving a foam roller back and forth on the posterior thigh muscle of the right leg. Results: There were statistically significant changes in the stiffness of the biceps femoris and the flexibility of the hamstring muscles. Conclusion: Self-myofascial release with foam rolling is an effective intervention method for increasing hamstring flexibility and ROM, and it is recommended as effective to perform self-myofascial release for three to four sessions to maintain normal muscle tone and stiffness of the hamstring muscles.