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PCR-based species identification method using unique genetic markers for invasive drywood termites (Cryptotermes domesticus), domesticus termites (Reticulitermes speratus), and kanmon termites (Reticulitermes kanmonensis)
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- URLhttps://db.koreascholar.com/Article/Detail/433435
This study focuses on developing diagnostic compositions, kits, and information provision methods for identifying species-specific genes in domestically residing Reticulitermes speratus and Reticulitermes kanmonensis, as well as the recently introduced Cryptotermes domesticus. The core innovation of this invention lies in the utilization of species-specific genetic markers to facilitate rapid and accurate species identification using a PCR (polymerase chain reaction)-based diagnostic technique. This approach enables swift identification of termites at quarantine stages, contributing to efficient management of imported goods and minimizing ecological and economic damages caused by termites. Through genome analysis of termites, this research has identified candidate species-specific genetic markers, developed diagnostic compositions and kits based on these markers, and proposed a rapid diagnostic method capable of determining termite species within a day, optimally within three hours. This invention provides a groundbreaking tool for termite management and research, significantly contributing to pest control and biodiversity conservation efforts.
