애기뿔소똥구리 (Copris tripartitus)는 배설물 분해를 통해 환경정화와 생태계 균형에 기여하는 것으로 알려져 있다. 그러나 무분별한 농약 사용과 서식지 파괴로 인해 개체수가 감소되고 있어 2017년 환경부에서 애기뿔소똥 구리를 멸종위기 야생생물 Ⅱ급으로 지정하였다. 애기뿔소똥구리의 미토콘드리아 유전자를 활용한 선행연구가 발표되었지만 유전자원 확보는 여전히 미비한 실정이다. 이번 연구는 애기뿔소똥구리의 성장, 면역 및 생식과 관련된 유전 정보를 확보하기 위해 Illumina HiSeq 4000 platform을 활용하여 전사체 분석을 실시하였다. Illumina HiSeq 4000을 통해 확보된 전사체 데이터를 Trinity 프로 그램을 통해 de novo assembly를 진행하여 contigs를 생성하였다. 생성된 contigs를 TGICL 프로그램을 통해 clustering 하여 unigenes을 확보하였다. 이후 확보된 unigenes는 PANM DB 및 Swiss-Prot, KOG, InterProScan, GO, KEGG를 기반으로 한 BLASTx를 사용하여 annotation을 진행하였다. 25,106개의 unigene 중에서 23,289개가 PANM DB에 annotation 되었으며, Swiss-Prot, InterProScan에서는 각각 19,660개, 13,545개의 unigene이 annotation 되었다. KOG 분석에서는 ‘general function prediction only’ 범주에서 높은 비율로 나타났으며, GO 분석에서는 ‘Molecular Function’ 카테고리에서 가장 많이 annotation 되었다. KEGG 분석을 통해서는 ‘Environmental information processing’ 항목이 높은 발현을 보였다. 이번 연구를 통해 확보된 기능적 데이터는 야생에서의 보존 계획을 수립하는 데 있어 기초 자료로써 활용될 수 있을 것으로 사료된다.

• Hyeok Lee(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Hee Ju Hwang(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Bharat Bhusan Patnaik(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), PG Department of Biosciences and Biotechnology, Fakir Mohan University, India)
• Snigdha Baliarsingh(PG Department of Biosciences and Biotechnology, Fakir Mohan University, India)
• Hongray Howrelia Patnaik(PG Department of Zoology, BJB Autonomous College, Odisha)
• Min Kyu Sang(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Research Support Center (Core-Facility) for Bio-Bigdata Analysis and Utilization of Biological Resources)
• Jie eun Park(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Research Support Center (Core-Facility) for Bio-Bigdata Analysis and Utilization of Biological Resources)
• Hang Chul Cho(iLAB, INSILICOGEN, INC., Korea)
• Dae Kwon Song(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Research Support Center (Core-Facility) for Bio-Bigdata Analysis and Utilization of Biological Resources)
• Jun Yang Jeong(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Chan Eui Hong(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Yong Tae Kim(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Hyeon Jun Sin(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Liu Ziwei(Department of Biology, College of Natural Sciences, Soonchunhyang University)
• So Young Park(Biodiversity Research Team, Animal & Plant Research Department, Nakdonggang National Institute of Biological Resources)
• Se Won Kang(Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Korea)
• Heon Cheon Jeong(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR))
• Hong Seog Park(Research Institute, GnC BIO Co., LTD.)
• Yeon Soo Han(College of Agriculture and Life Science, Chonnam National University)
• Yong Seok Lee(Department of Biology, College of Natural Sciences, Soonchunhyang University, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Research Support Center (Core-Facility) for Bio-Bigdata Analysis and Utilization of Biological Resources)