Meristem culture (MC) is a technique for producing virus-free garlic plants with high vigor and productivity. We assessed the changes in the agronomic traits of “Namdo” garlic over several generations after the cultivation of MC-induced bulbils. We examined the plant height, leaf sheath length and diameter, leaf number, bulb weight and diameter, clove number, and bulb size distribution. Compared with that of the control, bulb weights of the first-generation bulbils cultivated for three and two years and the second-generation bulbils cultivated for one year increased by 8.7–27.2, 13.9–30.4, and 36.6–46.9%, respectively. In three and two-year cultivation of the firstgeneration bulbils and one-year cultivation of the second-generation bulbils, the proportions of extra-large-sized bulb weight in meristem-cultured plants were 16.2–38.6, 24.0–35.8, and 27.1–51.1%, respectively, whereas that of the control was 7.6%. Thus, the first-generation bulbils can be cultivated for three years to renew the seed bulbs while maintaining productivity.

Introduction
Materials and Methods
    1. Plant materials and cultivation
    2. Collection and planting of bulbils
    3. Observation of agronomic traits
    4. Data analysis
Results and Discussion
    1. Plant and bulb traits
    2. The distribution of bulb sizes
Acknowledgments
References

• Binod Prasad Luitel(Post-doctoral Researcher, Allium Vegetable Research Center, National Institute of Horticultural and Herbal Science, Rural Development Administration, Muan, Jeollanam-Do, 58545, Korea, Senior Scientist, National Horticulture Research Center, Nepal Agricultural Research Council, Lalitpur, Bagmati Province, 5459, Nepal)
• JiWon Han(Researcher, Allium Vegetable Research Center, National Institute of Horticultural and Herbal Science, Rural Development Administration, Muan, Jeollanam-Do, 58545, Korea)
• Soohyun Kang(Researcher, Allium Vegetable Research Center, National Institute of Horticultural and Herbal Science, Rural Development Administration, Muan, Jeollanam-Do, 58545, Korea)
• Min-Seon Choi(Researcher, Allium Vegetable Research Center, National Institute of Horticultural and Herbal Science, Rural Development Administration, Muan, Jeollanam-Do, 58545, Korea) Corresponding author