The objective of this study was to investigate the anticancer effects of EMPS (edible mushroom mycelium polysaccharide: Tremella fuciformis) in animal models with colorectal cancer induced by AOM/DSS. The experimental groups consisted of Nor (normal), NC (AOM/DSS), EMPS (EMPS 50, EMPS 100), and PC (Fluorouracil). The NC group had the highest number of colon tumors, whereas it was observed that tumor occurrence was significantly reduced in the EMPS consumption group. The expression of Bcl-2, an apoptosis inhibitor, was significantly lower in the EMPS 50 & 100 and PC groups. On the other hand, the mRNA gene expression of Bax, a factor that induces apoptosis, was significantly higher in the EMPS 50 & 100 and PC groups compared to the NC group. The mRNA expression levels of TNF-α and COX-2 significantly increased in the NC group, but showed a significant decrease in the EMPS and PC groups, indicating inhibition of the cancer-promoting response of cells. At the phylum level of the mice's intestinal microbial composition, the proportion of Bacteroidetes tended to decrease, while the proportion of Firmicutes tended to increase with EMPS administration. This suggests that changes in the gut microbiota caused by inflammation can be influenced by dietary intake.

서 론
재료 및 방법
    1. 실험용 동물 준비 및 실험군 구성
    2. 대장암 유도
    3. 실험동물의 체중 변화량 측정 및 종양갯수 측정
    4. 대장조직의 병리학적 검사 결과
    5. 조직 내 mRNA 발현 측정(RT-qPCR)
    6. 조직 내 단백질 발현 측정 (Western Blot)
    7. 마우스 대장의 microbiota의 NGS 분석
    8. 통계 처리
결과 및 고찰
    1. 실험동물의 체중 변화
    2. 장기무게의 변화
    3. 대장길이 및 대장무게/길이 비율
    4. 대장조직 내 종양개수
    5. 대장 조직의 병리학적 분석
    6. RT-qPCR로 대장 조직 내 mRNA 발현 측정
    7. Western blot으로 대장 조직 내 단백질 발현 측정
    8. 장내 미생물 분석
요약 및 결론
감사의 글
References

• 공승식(호서대학교 벤처대학원 융합공학과 박사과정 학생, 호서대학교 보건산업연구소 연구원) | Seaung Sik Kong (Ph.D. Student, Dept. of Convergence Technology, Graduate School of Venture, Hoseo University, Seoul 06724, Korea, Researcher, Institute of Health Industry, Hoseo University, Seoul 06724, Korea)
• 강순아(호서대학교 벤처대학원 융합공학과 교수, 호서대학교 보건산업연구소 소장) | Soon Ah Kang (Professor, Dept. of Convergence Technology, Graduate School of Venture, Hoseo University, Seoul 06724, Korea, Director, Institute of Health Industry, Hoseo University, Seoul 06724, Korea) Corresponding author