This perspective article delves into the evolving landscape of non-viral vectors for efficient CRISPR delivery, addressing the challenges associated with viral vectors and highlighting the potential of carbon-based nanomaterials as promising alternatives. The article underscores the importance of design strategies in enhancing the interactions between CRISPR components and carbon-based nanomaterials. Various design approaches are explored, including the incorporation of modified nanoparticles between carbonic layers and the creation of unique morphologies to facilitate optimal CRISPR interactions. Specific case studies are presented to exemplify the effectiveness of carbon-based nanomaterials in CRISPR delivery. This perspective sheds light on the dynamic field of non-viral CRISPR delivery vectors, emphasizing the significance of design strategies and showcasing the promising outcomes achieved through the utilization of carbon-based nanomaterials. The provided insights contribute to the ongoing efforts to develop efficient and safe methods for gene delivery and therapy.

Carbon-based nanomaterials for CRISPRCas delivery: a perspective on the design approach
    Abstract
        Graphical abstract
    1 Introduction
    2 Design approach
    3 Future perspectives
    4 Conclusion
    Acknowledgements 
    References

• Moein Safarkhani(Department of Biological Sciences and Bioengineering, NanoBio High‑Tech Materials Research Center, Inha University, 100 Inha‑Ro, Incheon 22212, Republic of Korea, School of Chemistry, Damghan University, 36716‑45667 Damghan, Iran)
• Mohammad Reza Saeb(Department of Pharmaceutical Chemistry, Medical University of Gdańsk, J. Hallera 107, 80‑416 Gdańsk, Poland)
• Jeong‑Hwan Lee(Department of Materials Science and Engineering, Inha University, Incheon 22212, Republic of Korea)
• Yun Suk Huh(Department of Biological Sciences and Bioengineering, NanoBio High‑Tech Materials Research Center, Inha University, 100 Inha‑Ro, Incheon 22212, Republic of Korea) Corresponding author
• Navid Rabiee(Centre for Molecular Medicine and Innovative Therapeutics, Murdoch University, Perth, WA 6150, Australia, School of Engineering, Macquarie University, Sydney, NSW 2109, Australia)