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Construction of graphene/AuNPs based amperometric immunosensor for detecting bladder cancer biomarker apolipoprotein A1 KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/435109
This paper presents the construction and characterization of an amperometric immunosensor based on the graphene/gold nanoparticles (AuNPs/GO) nanocomposite for the detection of the bladder cancer biomarker, apolipoprotein A1 (Apo-A1). The morphological analysis of the AuNPs/GO nanocomposite demonstrated an almost spherical shape of AuNPs and the successful coverage of their surface by graphene oxide. An increased G peak and decreased D peak after the association of AuNPs with GO, implied a reduction in graphene defects. The X-ray photoelectron spectroscopy (XPS) indicated a significant decrease in the quantity of oxygen-containing functional groups in the AuNPs/GO nanocomposite, as compared to the original GO. Furthermore, the developed sensor demonstrated commendable sensitivity and selectivity, with a wide linear range for Apo-A1 detection. Importantly, the immunosensor exhibited remarkable stability over a period of 14 days, signifying its potential for practical applications.
Abstract
1 Introduction
2 Experimental
2.1 Materials
2.2 Preparation of GO
2.3 Preparation of AuNPsGO nanocomposite
2.4 Preparation of immunosensor
2.5 Determination of Apo-A1
3 Results and discussion
4 Conclusion
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