Current distribution of Korean Cottus pollux was investigated by conducting field surveys and environmental DNA analysis in September to November 2022 and March to May 2023. As a result of the field survey, the presence of C. pollux was confirmed in the uppermost stream of Hyeongsangang River, as well as in Deokdongcheon, Jeolgolcheon, and Hoamcheon Streams. The main habitat of C. pollux was the riffle area in the upper reaches of streams with clean water and boulder-cobble bottoms. As a result of environmental DNA analysis, the samples determined to be positive included all streams in which the presence of C. pollux was confirmed in the field survey. In addition, Namcheon and Singwangcheon Streams were determined to be positive, indicating potential as its habitats. Since C. pollux has a narrow distribution area and a small population size, continuous monitoring and conservation measures are required to immediately respond to damage caused by typhoons and river works, which are the main causes of habitat disturbance, in order to maintain a stable population.

서 론
재료 및 방 법
    1. 현장 조사
    2. 환경 DNA 분석
결 과
    1. 현장 조사
    2. 환경 DNA 분석
고 찰
적 요
REFERENCES

• 윤봉한(㈜한국생태네트워크) | Bong Han Yun (Institute of Korea Eco-Network, Daejeon 34028, Republic of Korea)
• 김용휘(㈜바이오티엔에스) | Yong Hwi Kim (bioTNS, Daejeon 34183, Republic of Korea)
• 이호성(순천향대학교 생명과학과) | Ho Sung Lee (Department of Biology, Soonchunhyang University, Asan 31538, Republic of Korea)
• 서은선(㈜자연환경복원연구원) | Eun Seon Seo (Natural Environmental Restoration Institute, Daejeon 34410, Republic of Korea)
• 이수형(㈜자연환경복원연구원) | Sue Hyeung Lee (Natural Environmental Restoration Institute, Daejeon 34410, Republic of Korea)
• 방인철(순천향대학교 생명과학과) | In-Chul Bang (Department of Biology, Soonchunhyang University, Asan 31538, Republic of Korea) Corresponding author