This study evaluated the virucidal efficacy of a chlorine dioxide (ClO2) gas-generating fumigation disinfectant consisting of sodium chlorate solution (25% sodium chlorate) and reaction solution against avian influenza virus (AIV). After AIV suspensions had been deposited on stainless steel carriers, the 9 dried carriers were exposed to the fumigant (sodium chlorate solution: 8.5, 17, 34, 50, and 100 mL) in a 25-m³ test room for 2, 3, and 4 h, respectively. Thereafter, all carriers were submerged in a neutralizing solution (20% fetal bovine serum) to scrape off the surviving viruses, and the respective suspensions were diluted. Each diluent was inoculated into the allantoic membrane of five 10-day-old embryos. After incubation for 5 days at 37℃, AIV viability in the collected allantoic fluids was examined, and the egg infectious dose 50 (EID50) was calculated. When the carrier was exposed to ClO2 gas generated from reacting 34 mL of the fumigant for 3 h, the AIV titer reduced by more than 104.0 EID50/carrier compared to the control, which was exposed to the fumigant without inoculation of AIV suspension. In addition, the control was non-toxic to the embryos.

서 론
재료 및 방법
    훈증 소독제
    공시바이러스 및 배양
    AIV 회수율 조사
    담체준비 및 설치
    이산화염소 농도 측정 및 소독제 훈증
    담체 중화반응 및 AIV 감염력 측정
    소독제 유효용량 및 권장용량 설정
결 과
    건조 담체 중 AIV 회수율
    ClO2 농도 변화
    훈증 소독제 노출 담체의 AIV 역가
    훈증 소독제의 유효용량 및 권장용량
고 찰
REFERENCES

• Chun-Nam Cha(Department of Industrial and Systems Engineering, Gyeongsang National University, Jinju 52828, Korea)
• Suk Kim(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Jin-Kyung Kim(Department of Dental Hygiene, Daegu Health College, Daegu 41453, Korea) Corresponding author
• Hu-Jang Lee(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea) Corresponding author