본 연구는 햄프씨드오일 (Cannabis sativa L. seed oil, CSO)와 햄프씨드오일 복합물 (Cannabis sativa L. seed oil complex, CSOC)의 비교분석을 통해 항염증 소재로서의 가능성을 평가하였다. CSO와 CSOC의 항염증 효과는 lipopolysaccharide (LPS)-induced RAW264.7 모델을 통해 확인하였다. Lipoxygenase inhibition activity를 통해 지질산화억제를 확인한 결과, CSO는 억제되지 않았지만 COSC는 70% 이상 억제되었다. MTT assay를 통해 세포독성을 확인해본 결과, CSO는 세포독성을 보이지 않았지 만 CSOC는 200 μg/ml 이상 농도에서 세포독성을 보였다. LPS로 유도된 RAW264.7에서 inducible nitric oxide synthase (iNOS)의 발현과 nitric oxide (NO)의 생산은 CSOC가 CSO보다 현저하게 억제하였다. 또한, cyclooxygenas (COX)-2의 발현과 prostraglandin E2 (PGE2)의 생성을 확인해본 결과에서도 CSOC 가 CSO보다 현저하게 억제하였다. 본 연구를 통해, CSOC가 CSO보다 우수한 항염증 효과를 가지며, 항 염증 소재로 활용 가능성이 있음을 확인했다.

This study evaluated the potential of hemp seed oil (Cannabis sativa L. seed oil, CSO) and hemp seed oil complex (Cannabis sativa L. seed oil complex, CSOC) as an anti-inflammatory material through comparative analysis. Anti-inflammatory effects of CSO and CSOC were confirmed through lipopolysaccharide (LPS)-induced RAW264.7 model. As a result of confirming the inhibition of lipid oxidation through lipoxygenase inhibitory activity, CSO was not inhibited, but COSC was inhibited by more than 70%. As a result of confirming cytotoxicity through MTT analysis, CSO did not show cytotoxicity, but CSOC showed cytotoxicity at over 200μg/ml. In LPS-induced RAW264.7, the expression of inducible nitric oxide synthase (iNOS) and the production of nitric oxide (NO) were significantly inhibited by CSOC compared to CSO. Additionally, CSOC significantly inhibited the expression of cyclooxygenase (COX)-2 and the production of prostaglandin E2 (PGE2). Through this study, we confirmed that CSOC has superior anti-inflammatory effects than CSO and has the potential to be used as an anti-inflammatory material.

1. 서 론
2. 실 험
    2.1. 실험재료
    2.2. 오일 추출 및 복합물 제조
    2.3. Lipoxygenase inhibition activity 측정
    2.4. 세포주 및 배양 조건
    2.5. Cell viability assay
    2.6. Nitric oxide (NO) assay
    2.7. 단백질 정량 및 immunoblotting
    2.8. RNA 분리 및 quantitative real-timePCR (qPCR)
    2.8. Enzyme-linked immunosorbent assay(ELISA)
    2.9. 통계분석
3. 결과 및 고찰
    3.1. CSOC와 CSO의 cell viability
    3.2. CSOC와 CSO의 Lipoxygenase (LOX)inhibition activity
    3.3. CSOC와 CSO의 morphology analysis
    3.4. CSOC와 CSO의 anti-inflammatoryeffects
4. 결 론
사 사
References

• 김채현(경성대학교 화장품학약리학과) | Chae-Hyun Kim (Department of Cosmeceutical Science, Kyungsung University, Busan 48434, Republic of Korea)
• 김세기(대구가톨릭대학교 제약공학과) | Se Gie Kim (Department of Pharmaceutical Engineering, Daegu Catholic University, Gyeongsan 38430, Republic of Korea)
• 장영아(대구한의대학교 화장품학과) | Young-Ah Jang (Department of Cosmetic Science, Daegu Haany University, Gyeongsan 38610, Republic of Korea)
• 권용진(경성대학교 화장품학약리학과, 경성대학교 화장품학과) | Yong-Jin Kwon (Department of Cosmeceutical Science, Kyungsung University, Busan 48434, Republic of Korea, Department of Cosmetic Science, Kyungsung University, Busan 48434, Republic of Korea) Corresponding author