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Antioxidant activity of 3,5-dicaffeoyl-epi-quinic acid (DEQA) from the halophyte Atriplex gmelinii KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/435735
pp.447-458
In this study, the antioxidizing effect of 2,3-dicaffeoyl-epi-quinic acid (DEQA) was investigated. The antioxidant activity was evaluated by measuring the scavenging effect on DPPH radical and peroxynitrite and the reducing power on ferric ion. DEQA showed a scavenging effect and reducing power comparable to vitamin C used as a positive control. Also, DEQA effectively inhibited production of intracellular reactive oxygen species (ROS) in HT-1080 cells, showing the scavenging ratio of 43.8% even at 10 μM concentration of DEQA after 2 hours in HT-1080 treated with H2O2. In addition to this, DEQA inhibited the production of nitric oxide (NO) very effectively in Raw 264.7 cells. The above results suggest that DEQA has the potential to be developed as a natural antioxidant.
2. Materials and methods
2.1. Reagents and materials
2.2. Collection, extraction, fractionation,and isolation
2.3. Cell culture
2.4. Scavenging activity against1,1-diphenyl-2-picrylhydrazyl (DPPH)radical
2.5. Scavenging activity against peroxynitrite
2.6. Measurement of ferric ion reducingantioxidant power (FRAP)
2.7. Measurement of cell viability usingMTT assay
2.8. Measurement of intracellular ROS
2.9. Measurement of NO production
2.10. Statistical analysis
3. Results and Discussion
3.1. Scavenging activity against DPPHradical
3.2. Scavenging activity against peroxynitrite
3.3. Measurement of ferric ion reducingantioxidant power (FRAP)
3.4. Measurement of cell viability usingMTT assay
3.5. Measurement of scavenging activityfor intracellular ROS
3.6. Measurement of inhibitory effectagainst NO production
4. Conclusion
Acknowledgments
References
