논문 상세보기
Effects of culture conditions on the adenosine production in submerged culture of Paecilomyces tenuipes KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/436760
pp.73-80
We studied the effects of initial pH, different nitrogen sources, and cultivation methods (shake flask and static culture) on biomass production, exopolysaccharides (EPS), and adenosine by Paecilomyces tenuipes. Relatively low pH levels were optimal for mycelial growth and EPS production. Yeast extract was the most effective organic nitrogen source for EPS production, whereas soybean extract was the best for adenosine production. A high C/N ratio was beneficial for adenosine production; however, excessively high C/N ratios reduced adenosine production. Static fermentation significantly increased adenosine production. A Box-Behnken design was used to optimize adenosine production; the optimal conditions for adenosine production by P. tenuipes were pH 7.0, soybean concentration of 3%, and a static culture period of 20 days, with the maximum adenosine production of 141.10 mg/L (predicted value: 128.05 mg/L).
MATERIALS AND METHODS
Mycelium and seed culture
Flask culture conditions
Determination of mycelia dry weight, pH and residual sugar
Measurements of exopolysaccharide
Measurement of adenosine by HPLC
Response surface methodology (RSM), the Box–Behnken design
Statistical Analyses
RESULTS AND DISCUSSION
Effect of pH on cell growth and the production of metabolites
Effect of nitrogen sources on cell growth and the production of metabolites
Shake-flask and static culture of P. tenuipes
Optimization of adenosine production by RSM (Box–Behnken design)
ACKNOWLEDGEMENTS
REFERENCES
