The present study was conducted to investigate effects of rabbit meat extract on energy metabolism and muscle differentiation in C2C12 myotubes. Water extract of rabbit meat (10, 50, 100, and 200 μg/ml) was used to treat differentiated C2C12 cells. Reverse transcriptase polymerase chain reaction (RT-PCR) and western blot analysis were used to determine mRNA or protein levels of energy metabolism-related genes. Total adenosine triphosphate (ATP) content was also measured. Treatment with rabbit meat extract significantly increased expression levels of muscle differentiation markers (myogenin and myosin heavy chain) and mitochondrial biogenesis regulators (PGC1α, NRF1, and TFAM) in C2C12 myotubes compared to non-treated control. Additionally, rabbit meat extract activated phosphorylation of AMPK and acetyl-coA carboxylase (ACC). Rabbit meat extract significantly increased ATP contents in myotubes. These results suggest that rabbit meat extract has the potential to improve energy metabolism in skeletal muscles.

Abstract
서 론
재료 및 방법
    1. 토끼추출물 제조
    2. 세포 배양 및 근육 분화
    3. 세포독성 평가
    4. Quantitative real-time polymerase chain reaction(qRT-PCR)
    5. Western blot
    6. ATP assay
    7. 통계 분석
결과 및 고찰
    1. 세포독성
    2. 근육분화에 대한 효과
    3. 골격근 세포에서 에너지대사에 대한 효과
    4. 골격근 세포에서 토끼고기 추출물의 AMPK/ACCsignaling pathway에 대한 효과
    5. 골격근 세포에서 ATP 생산에 대한 효과
요약 및 결론
감사의 글
References

• 배인선(농촌진흥청 국립축산과학원 축산물이용과 농업연구사) | In-Seon Bae (Researcher, Animal Products Utilization Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea) Corresponding author
• 이정아(농촌진흥청 국립축산과학원 축산물이용과 박사과정) | Jeong Ah Lee (Doctoral Student, Animal Products Utilization Division, National Institute of Animal Science, Rural Development Administration,Wanju 55365, Korea)
• 박원서(농촌진흥청 국립축산과학원 축산물이용과 농업연구사) | Won-Seo Park (Researcher, Animal Products Utilization Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea)
• 유자연(농촌진흥청 국립축산과학원 축산물이용과 농업연구사) | Jayeon Yoo (Researcher, Animal Products Utilization Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea)
• 함준상(농촌진흥청 국립축산과학원 축산물이용과 농업연구관) | Jun-Sang Ham (Senior Researcher, Animal Products Utilization Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea)
• 서강민(농촌진흥청 국립축산과학원 동물복지연구팀 연구원) | Kangmin Seo (Researcher Assistant, Animal Welfare Research Team, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea)
• 김기현(농촌진흥청 국립축산과학원 동물복지연구팀 농업연구사) | Ki Hyun Kim (Researcher, Animal Welfare Research Team, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea)