Oxidative stress caused by reactive oxygen species (ROS) is a key mechanism of skin aging, and the use of antioxidants is an effective strategy to prevent the symptoms associated with ROS-induced skin aging. The components rich in polyphenolic compounds with antioxidative activity were identified by fractionating an aqueous ethanolic extract of Gryllus bimaculatus (AE-GBE) using solvent using hexane, chloroform, ethyl acetate, butanol, and water. Their ability to migrate H2O2-induced oxidative stress in human dermal fibroblasts (HDFs) was then evaluated. The butanol fraction of AE-GBE had the highest polyphenol content and antioxidant effect, followed by the ethyl acetate and water fractions, suggesting that the likely antioxidant components are polar components. Furthermore, the butanol, ethyl acetate, and water fractions effectively reduced intracellular ROS production and DNA damage in HDF cells caused by H2O2. Overall, these findings suggest that the butanol fraction of AE-GBE shows promise as a natural insect-derived antioxidant material, capable of suppressing oxidative stress by showing a stronger antioxidant effect under H2O2 stimulation than the other fractions.

ABSTRACT
I. 서 론
II. 연구 내용 및 방법
    1. 시약
    2. AE-GBE 조 추출물의 조제 및 용매 분획(Solvent partition)
    3. 총 페놀 함량 및 항산화 활성 측정
    4. 세포 배양
    5. 세포 생존도 측정
    6. 세포 내 활성산소 측정
    7. ELISA assay
    8. 통계처리
III. 결과 및 고찰
    1. AE-GBE의 용매 분획별 총 폴리페놀 함량 및 DPPH 라디칼 소거 활성
    2. AE-GBE 용매별 분획물의 산화스트레스에 의한 세포독성보호 효과
    3. AE-GBE 분획물의 산화스트레스 유도에 대한 IntracellularROS 소거 활성 및 DNA 손상 저해 효과
IV. 요약 및 결론
저자정보
감사의 글
Conflict of Interest
References

• 김경(을지대학교 식품영양학과) | Kyong Kim (Department of Food and Nutrition, Eulji University)
• 오윤신(을지대학교 식품영양학과) | Yoon Sin Oh (Department of Food and Nutrition, Eulji University) Corresponding author