Cold-pressed rapeseed oil (CPRO) is a rich source of bioactive components, but it is more susceptible to deterioration due to absence of chemical refining. Proper storage conditions are essential to maintain oil quality. This study evaluated the quality properties of seed-stored oil (SSO) and oil-stored oil (OSO) under various storage conditions. Both the seeds and the CPRO obtained from the ‘Jungmo7001’ and ‘Yuryeo’ varieties were stored for 24 months at 4℃, 25℃, and ambient temperature (AT). After 24 months, the acid value (AV) at 4℃ was lower than at 25℃ and AT. Additionally, the AV increased by 3.0-fold in SSO and 1.9-fold in OSO at AT compared to initial storage levels. OSO was found to be relatively more stable against oxidation than SSO. The canolol content in SSO increased with both storage temperature and period, but this was not observed in OSO. However, no changes in fatty acid composition or tocopherol content were noted based on the storage conditions, indicating that these properties remained relatively stable during storage. These results provide foundational data for the stable distribution of CPRO.