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Development of an F2 Population and GBS Analysis for Identifying QTL Associated with Resistance to Bacterial Wilt in Pepper KCI 등재
고추 풋마름병 저항성 관련 QTL 탐색을 위한 F2 집단 구성과 GBS 분석
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/438487
pp.414-422
Pepper is one of the most important vegetables in South Korea. It is a key ingredient in kimchi, the nation’s staple dish, and serves as the primary raw material for producing gochujang, a commonly used condiment in Korean cooking. As a result, numerous pepper varieties have been developed, including those that yield more fruit or have milder pungency. However, farmers who grow peppers tend to prefer varieties that are resistant to pests and diseases. Bacterial wilt (BW) is one of the most devastating diseases affecting peppers and is transmitted through the soil. To breed pepper varieties resistant to bacterial wilt using molecular breeding techniques, it is essential to first identify the Quantitative Trait Locus (QTL) that confers resistance to this disease. This requires conducting locus analysis with resistant cultivars. In this study, an F2 population was developed by selfing F1 hybrids, which were obtained by crossing a resistant cultivar with a susceptible cultivar, to identify QTLs associated with bacterial wilt resistance. Genotyping-by-Sequencing (GBS) analysis will be performed using the F2 population, and the results will be utilized for QTL mapping.
INTRODUCTION
MATERIALS AND METHODS
Plant materials and population development
Preparation of bacterial strain
Inoculation method
DNA extraction
GBS analysis
RESULT
Evaluation of resistant degrees to bacterial wilt inpepper
DISCUSSION
적 요
ACKNOWLEDGMENTS
REFERENCES
