This study developed an unmanned autonomous moving algae collection device (HAMA-bot) to remove high-density algae concentrated in the waterfront of urban agricultural reservoirs, and analyzed the effect of algae removal after field application to medium-sized urban reservoir. The algae reduction effect (Chl-a) of the study site in the reservoir by the HAMA-bot operation showed an average 18.5% higher in the treatment area compared to the control area before operation, while the average reduction of 24% in the treatment area after operation. In addition, the Chl-a removal rate, which directly analyzed the influent and effluent of HAMA-bot, showed a very high level with an average of 96.9% (94.7~99.2%). Currently, it is optimized for urban reservoirs and manufactured on a small scale, but it is a useful tool that can be applied on a large scale to large dams and rivers, and it is considered that the field applicability would be improved with the optimized scale.