Because intact FMDV particles (146S) are often unstable in vitro, stabilizing foot-and-mouth disease virus (FMDV) antigens remains a key challenge in studying viral charateristics. Therefore, finding optimal condition to stabilize the FMDV is essential. In this study, we investigated formulations and potentials of several stabilizers such as appropriate buffer, excipients, and storage conditions to enhance the stability of 146S. Inactivated FMDV O-Jincheon (O-JC) was dissolved in various buffer formulations, and stored at 4℃ for two months to evaluate quantity of 146S at every 2-week interval. Among phosphate buffered saline (PBS), Tris buffered saline (TBS), HEPES buffered saline (HBS), and MOPS buffered saline (MBS), PBS showed more effective 146S stabilization that showed 1.3-1.6 fold higher 146S fraction than TBS, HBS, and MBS after storage for 2 weeks. However, constant dissociations of 146S were observed in all formulations at 8 weeks. Compared with other FMDVs, A22 Iraq and SAT-1, in PBS, O-JC proved to be the least stable in PBS. A variety of excipients including carbohydrate, sugar alcohol, cryo-protectant were tested for the capability in protecting O-JC from dissociation. By adding 4-8% sucrose, more than 60% of 146S fractions were maintained at 8 weeks, those were at least 1.8 fold higher than the PBS-only control. Addition of 1% β-cyclodextrin showed synergistic enhancement in O-JC stability. As the results of this study, it could be suggested that the PBS-based buffer together with 4-8% sucrose + 2% sorbitol or 2% sucrose + 2% sorbitol + 1% β-cyclodextrin could help the better stability of the O-JC in vaccine preparation.

Abstract
서 론
재료 및 방법
    세포 및 바이러스 준비
    바이러스 증식
    바이러스 항원 제조
    완충용액 및 첨가제
    146S 항원 측정
결 과
    항원별 안정성 비교
    pH 완충용액별 146S 안정화 효과 비교
    PBS 기반 Sucrose 및 Sorbitol 혼합 조성에 따른 O-JC146S 항원 안정성 평가
    PBS 기반 1:1 고정 농도의 여러 추가 첨가제 조성에 따른O-JC 146S 항원 안정성 평가
고 찰
감사의 글
REFERENCES

• Sungho Shin(Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea)
• Yong Bin Kim(Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea)
• Seung Heon Lee(Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea)
• Hye Jun Jo(Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea)
• Seon Woo Kim(Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea)
• Jong-Hyeon Park(Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea)
• Jaejo Kim(Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea) Corresponding author