Endosymbionts of the genus Buchnera, which belong to γ-Proteobacteria, reside in specialized cells known as bacteriocytes. In this announcement, we present the complete genome sequence of Buchnera aphidicola (Aphis gossypii) isolated from Aphis gossypii with Plantago asiatica. The genome spans 628,098 base pairs and has a GC content of 25.4%. A total of 589 CDs, 32 tRNAs, and 3 rRNAs were predicted. Interestingly, the evolutionary rate of the endosymbiont's genome, inferred from intraspecific variations, may be slower than that of the host's mitochondrial genome.