Gynura bicolor is a medicinal plant recognized for its antioxidant potential and natural pigments. This study aimed to evaluate antioxidant activities and bioactive compounds’ contents of G. bicolor extracts and solvent fractions prepared using different drying methods and solvent partitioning. Ethanol extracts were obtained from freeze-dried (FD) and hot-air-dried (HD) samples, followed by sequential partitioning with n-hexane (n-Hex), methylene chloride (MC), ethyl acetate (EA), and n-butanol (n-BuOH). Total polyphenol (TP) and flavonoid (TF) contents were measured. Antioxidant activities were assessed via DPPH and ABTS radical scavenging assays. The FD-EtOH extract showed the highest TP (100.8±0.29 mg TAE/mL) and TF (4.72±0.03 mg QE/mL) contents. EA and n-BuOH fractions exhibited significantly higher antioxidant activity than other fractions, suggesting that bioactive compounds were effectively concentrated through selective solvent extraction. These findings indicate that drying and solvent fractionation can significantly affect antioxidant functionality and phytochemical yield. G. bicolor may serve as a promising natural antioxidant and pigment source for functional food and beverage development.

Abstract
서 론
재료 및 방법
    1. 실험재료-재료 및 시약
    2. 건조,추출 및 분획
    3. 항산화 활성
    4. 통계처리
결과 및 고찰
    1. DPPH 라디칼 소거 활성
    2. ABTS 라디칼 소거 활성
    3. 총 폴리페놀 및 플라보노이드 함량 분석
    4. 총 안토시아닌 함량 분석
    5. 상관관계분석
    6. EtOH 추출물의 분획 수율 및 용매 특성 해석
요약 및 결론
References

• 문해주(우석대학교 식품생명공학과 대학원생) | HaeJu Moon (Graduate Student, Dept. of Food and Biotechnology, Woosuk University, Wanju 55338, Korea)
• 오석흥(우석대학교 식품생명공학과 교수) | SukHeung Oh (Professor, Dept. of Food and Biotechnology, Woosuk University, Wanju 55338, Korea)
• 김준형(우석대학교 식품생명공학과 교수) | JunHyeong Kim (Professor, Dept. of Food and Biotechnology, Woosuk University, Wanju 55338, Korea) Corresponding author