Gynura bicolor is a medicinal plant recognized for its antioxidant potential and natural pigments. This study aimed to evaluate antioxidant activities and bioactive compounds’ contents of G. bicolor extracts and solvent fractions prepared using different drying methods and solvent partitioning. Ethanol extracts were obtained from freeze-dried (FD) and hot-air-dried (HD) samples, followed by sequential partitioning with n-hexane (n-Hex), methylene chloride (MC), ethyl acetate (EA), and n-butanol (n-BuOH). Total polyphenol (TP) and flavonoid (TF) contents were measured. Antioxidant activities were assessed via DPPH and ABTS radical scavenging assays. The FD-EtOH extract showed the highest TP (100.8±0.29 mg TAE/mL) and TF (4.72±0.03 mg QE/mL) contents. EA and n-BuOH fractions exhibited significantly higher antioxidant activity than other fractions, suggesting that bioactive compounds were effectively concentrated through selective solvent extraction. These findings indicate that drying and solvent fractionation can significantly affect antioxidant functionality and phytochemical yield. G. bicolor may serve as a promising natural antioxidant and pigment source for functional food and beverage development.