The postharvest quality of cut flowers often declines during transportation, especially under dry storage conditions. Therefore, incorporating preservative solutions during shipment is vital for extending the vase life of high-value cut flowers. Among commonly used additives, sucrose (Suc) and Floralife (FL), a commercial preservative, have demonstrated efficacy in maintaining vase life. Oxygen nanobubbles (O2NB) and ozone nanobubbles (O3NB), which are nanoscale gas-filled bubbles in aqueous media, have been proposed as antimicrobial agents for use in preservative solutions applied to cut flowers. This study examined the effects of seven preservative treatments on the postharvest performance of cut Cymbidium ‘Lovely King’ during simulated transport. Treatments included tap water (control), Suc, FL, O2NB, O2NB combined with Suc (O2NBS), O3NB, and O3NB combined with Suc (O3NBS). Cut stems were placed in floral water tubes filled with the respective solutions and stored in a cold chamber at 5°C for 7 days to simulate transport conditions. Among the treatments, O2NB resulted in the longest vase life of 21.0 days, significantly longer than that under O2NBS (14.3 days). Additionally, the O2NB solution exhibited the lowest bacterial count (4.9 log 10 CFU mL-1) compared with other treatment solutions. Stems treated with O3NB maintained the highest relative fresh weight throughout the experiment. Initial water uptake was 8.4 g and 7.6 g for the O3NB and O2NB treatments, respectively. These findings indicate that O2NB is an effective transport preservative for enhancing postharvest quality and extending the vase life of cut Cymbidium ‘Lovely King.’

Introduction
Materials and Methods
    Plant materials
    Experimental environments
    Preservative solutions
    Data collection and analysis
Results
    Vase life
    Bacterial counts and microscopy observation onvascular
    Relative fresh weight and water uptake
    Flower diameter
Discussion
Acknowledgments
References

• You Kyung Chung(Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women’s University, Seoul 01797, Korea)
• Yoon Jin Kim(Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women’s University, Seoul 01797, Korea) Corresponding author