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Evaluation of Pharmacological Properties of Nicotinamide Mononucleotide (NMN) for Cosmetic Applications KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/443396
pp.206-215
This study aimed to evaluate the anti-inflammatory, whitening, and anti-wrinkle effects of nicotinamide mononucleotide (NMN) and NMN-containing serum. To investigate the anti-inflammatory effects of NMN, a lipopolysaccharide (LPS)-induced RAW264.7 macrophage model was used. The results demonstrated that NMN exhibited no cytotoxicity at concentration up to 1%. Additionally, 1% NMN significantly inhibited nitric oxide (NO) production induced by LPS and suppressed the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), both of which are key inflammatory enzymes. Furthermore, to assess the whitening and anti-wrinkle effects of NMN, tyrosinase inhibition, elastase inhibition, and collagenase inhibition assays were conducted. The results showed that 1% NMN inhibited tyrosinase activity by 10.85%, elastase activity by 18.69%, and collagenase activity by 91.94%. These research results suggest that although NMN has a relatively limited whitening effect, it has significant potential as an anti-inflammatory and wrinkle-improving agent, suggesting its potential as a cosmetic material.
1. Introduction
2. Materials and methods
2.1. Reagents and antibodies
2.2. Measurement of tyrosinase inhibitoryactivity
2.3. Measurement of elastase inhibitoryactivity
2.4. Measurement of collagenaseinhibitory activity
2.5. Cell line and culture conditions
2.6. Cell viability
2.7. NO production
2.8. Immunoblotting
2.9. Stability evaluation
2.10. Statistical analysis
3. Results and discussions
3.1. Cell cytotoxicity and morphologicalchanges of NMN in RAW264.7 cells
3.2. Anti-inflammatory effects of NMN inLPS-induced RAW264.7 cells
3.3. Whitening and wrinkle improvementeffects of NMN
3.4. Stability evaluation of a serumformulation containing 1% NMN
4. Conclusion
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