최근 천연 유래 미백제에 대한 관심이 증가하고 있다. 천연 유래 미백성분은 ‘자연 유래’라 는 메시지를 소비자에게 호감을 주어 경쟁력을 높인다. 본 연구는 Xanthium strumarium seed 에탄올 추출물의 미백효능을 평가하였다. 그 결과 α-MSH(α-melanocyte stimulating hormone)로 유도한 B16F10에서 티로시나아제와 멜라닌 형성을 억제했다. 또한 핵심조절인자인 티로시나제, MITF (Microphthalmia-associated transcription factor), TRP-1(tyrosinase related protein-1), TRP-2 (tyrosinase related protein-2)에 관여함으로서 최종 멜라닌 생성을 저해하였다. 이러한 결과는 X. strumarium seed 에탄올 추출물의 미백 기능성 성분으로서의 가능성을 시사한다.

Recently, interest in natural whitening agents has been increasing. Natural whitening ingredients increase competitiveness by giving consumers a favorable message of ‘natural origin’. This study evaluated the whitening efficacy of Xanthium strumarium seed ethanol extract. As a result, tyrosinase and melanin formation were inhibited in B16F10 cells induced by α-MSH (α -melanocyte stimulating hormone). In addition, by involving key regulatory factors such as tyrosinase, MITF (Microphthalmia-associated transcription factor), TRP-1 (tyrosinase related protein-1), and TRP-2 (tyrosinase related protein-2), the final melanin formation was prevented. These results suggest the potential of X. strumarium seed ethanol extract as a whitening functional ingredient.

요 약
Abstract
1. Introduction
2. Materials and methods
    2.1. Materials
    2.2. Preparation of X. strumarium seedethanol extract
    2.3. Cell cytotoxicity ananlysis
    2.4. Measurement of tyrosinase inhibitionactivity and melanin synthesis
    2.5. Quantitative real-time PCR analysis
3. Results and Discussion
    3.1. Evaluation of the viability of X.strumarium seed extracts in B16F10cells
    3.2. Tyrosinase and melanin biosynthesisinhibitory activities of X. strumariumseed extracts
    3.3. Inhibitory effect of X. strumariumseed extract on melanin productionrelatedprotein and gene expression
    3.4. Discussion
4. Conclusion
References

• 김보애(목원대학교 보건안전대학 화장품학과, 부교수) | Bo Ae Kim (Department of Cosmetics, College of Health and Safety Science, Mokwon University, Daejeon, 35349, Republic of Korea) Corresponding author