This study examined the anti-inflammatory potential and metabolite profiles of Perilla frutescens var. frutescens leaf germplasm. The anti-inflammatory activity was assessed in vitro by measuring nitric oxide (NO) production and interleukin-8 (IL-8) secretion. Among the germplasm tested, PL7 and PL6 exhibited the strongest inhibitory effects on both NO and IL-8. Metabolite profiling, conducted using liquid chromatography-mass spectrometry (LC-MS), identified 60 compounds, primarily flavonoids and phenolic acids. Principal component analysis (PCA) grouped the samples into four distinct clusters: PL1–PL5, PL6, PL7, and PL8–PL10. Variable importance in projection (VIP) analysis highlighted 15 key metabolites (VIP>1.0), including tuberonic acid glucoside, caffeic acid, luteolin, and salicylic acid, which contributed to the separation of these groups. Heatmap visualization revealed distinct patterns of metabolite accumulation: vitexin and rosmarinic acid were abundant in PL1–PL5; vanillic acid-4-glucoside and prulaurasin were prominent in PL6; 5'-O-β-D-glucosylpyridoxine and esculin were concentrated in PL7; and luteolin and D-pantothenic acid were enriched in PL8–PL10. Further analysis identified key anti-inflammatory metabolites, such as apigenin-6,8-diglucoside, salicylic acid, and pinocembrin in PL6, along with quercetin-3-O-glucuronide, rosmarinic acid-3'-glucoside, and quercetin-3-O-glucoside in PL7. These findings highlight the functional diversity among perilla germplasm and their potential as valuable sources of anti-inflammatory food ingredients.

Abstract
서 론
재료 및 방법
    1. 실험 재료
    2. 추출물의 제조
    3. 세포 배양 및 세포 생존율 평가
    4. Nitric oxide(NO) 함량
    5. Interleukin-8(IL-8) 분비 억제 효능 평가
    6. LC-MS 기반 들깻잎의 비표적 대사체 분석
    7. 통계 분석
결과 및 고찰
    1. 들깻잎 추출물의 Nitric oxide(NO) 생성 억제 효과
    2. 들깻잎 추출물의 interleukin-8(IL-8) 분비 억제 효과
    3. LC-MS 기반 들깻잎의 비표적 대사체 분석
    4. 들깻잎 유전자원의 다변량 통계 분석
요약 및 결론
감사의 글
References

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• 조수연(농촌진흥청 국립식량과학원 식품자원개발부 연구원) | Soo Yeon Cho (Researcher, Dept. of Food Sciences, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Korea)
• 김현정(농촌진흥청 국립식량과학원 식품자원개발부 연구원) | Hyeon-jeong Kim (Researcher, Dept. of Food Sciences, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Korea)
• 유은애(농촌진흥청 국립농업과학원 농업유전자원센터 농업연구사) | Eunae Yoo (Researcher, National Agrobiodiversity Center, National Institute of Agricultural Science, Rural Development Administration, Jeonju 55365, Korea)
• 이수경(농촌진흥청 국립농업과학원 농업유전자원센터 농업연구사) | Sookyeong Lee (Researcher, National Agrobiodiversity Center, National Institute of Agricultural Science, Rural Development Administration, Jeonju 55365, Korea)
• 장환희(농촌진흥청 국립식량과학원 식품자원개발부 농업연구사) | Hwan-Hee Jang (Researcher, Dept. of Food Sciences, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Korea)
• 강해주(농촌진흥청 국립식량과학원 식품자원개발부 농업연구사) | Hae Ju Kang (Researcher, Dept. of Food Sciences, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Korea) Corresponding author