This study evaluates the structural stability of a hydrogen shut-off valve used in fuel cell electric vehicles (FCEVs) under extreme operating conditions, including high pressure and cryogenic temperatures. Using a one-way Fluid-Structure Interaction (FSI) analysis based on ANSYS CFX and Static Structural, the study simulates thermal and pressure loads on key components. The results show that the maximum equivalent stress occurs in the rod (361.22 MPa), while safety factors for all components remain above 2.11, confirming adequate structural integrity. In order to secure higher structural stability and reduce the weight of parts, attention should be paid to the selection of materials and improving the shape. The findings provide a valuable basis for improving the design reliability and optimization of hydrogen shut-off valves for future automotive applications. The leak tightness and durability tests of the hydrogen shut-off valve under cryogenic conditions verified its structural integrity, confirming its safety even after more than 5.2 million repeated operations.