The brix% of ASI 52822 resulted in the highest solid solubility. ASI 53399, ASI 50167, and ASI 52822 displayed high carbohydrate contents, whereas GBGA 01, GBGA 02, and ASI 52823 exhibited the half those values. The highest protein content was measured in ASI 52822. The antioxidant activity in the case of the hydrothermal method was lower than that for ascorbic acid, although it revealed more than 65% antioxidant activity. Furthermore, ASI 53399 and ASI 52822 exhibited antioxidant activities of 83.8% and 81.3%, respectively. To confirm the anticancer activity of the Ganoderma applanatum extract, we measured the original extract and also prepared a 10-time extract. The 10-time extract yielded strong anticancer activity, although the original extract did not exhibit any.

ABSTRACT
Introduction
Materials and Methods
    Preperation of G. applanatum
    Brix (%)
    Measurement of sum sugar
    Measurement of protein contents
    Measurement of Antioxidant (DPPH assay)
    Antitumor activity
Results and Discussion
    Brix%
    Total carbohydrate
    Protein contents
    Measurement of Antioxidant (DPPH assay)
    Anticancer activity
Conclusion
Acknowledgements
REFERENCES

• Woo-Sik Jo(Gyeongsangbuk-do Provincial Agricultural Research & Extension Service, Daegu 41404, Republic of Korea) Corresponding author
• Young-Hyun Rew(Gyeongsangbuk-do Provincial Agricultural Research & Extension Service, Daegu 41404, Republic of Korea)
• Seung-Chun Park(College of Veterinary Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea)