간행물
한국버섯학회지 KCI 등재 Journal of Mushrooms (J. Mushrooms)
- 발행기관 한국버섯학회
- 자료유형 학술지
- 간기 계간
- ISSN 1738-0294 (Print)2288-8853 (Online)
- 수록기간 2003 ~ 2025
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 525DDC 635
권호리스트/논문검색
제23권 제3호 (2025년 9월) 13건
1.
2025.09
구독 인증기관 무료, 개인회원 유료
Exosome-like particles (ELPs) derived from natural products have attracted considerable interest for drug delivery and therapeutic applications. Mushroom-derived exosome-like particles (MELPs) are an unexplored group with significant biomedical potential. Because medicinal and edible mushrooms are rich in bioactive compounds, such as polysaccharides, proteins, and phenolic acids, it is likely that their ELPs also carry these compounds during their biogenesis. Hence, MELPs containing these beneficial compounds, which exhibit favorable characteristics such as nanosize, stability, and cellular uptake properties, should be considered a novel potential natural nanocarrier. Here, we have detailed the current understanding of MELPs in terms of their biological functions, physicochemical characteristics, molecular cargo, and isolation methods. We have also highlighted their potential applications in cancer therapy, immune modulation, inflammation, and microbiome regulation. Moreover, we have outlined the challenges and future directions for standardizing the isolation protocol to yield large-scale production and for elucidating the mechanistic pathways. As MELP research advances, it may emerge as a next-generation nanocarrier in natural therapeutics and green nanomedicine.
4,000원
2.
2025.09
구독 인증기관 무료, 개인회원 유료
This review assessed the feasibility and effectiveness of chemical sterilization methods as alternatives to conventional physical sterilization methods, with a specific focus on mushroom substrate preparation. Physical sterilization methods, such as autoclaving, provides excellent sterilization efficacy, but are resource-intensive and costly, limiting accessibility for small-scale mushroom growers. This review systematically examined recent research on chemical sterilization agents, notably hydrogen peroxide (H2O2) and chlorine dioxide (ClO2), focusing on sterilization efficacy, biological efficiency (BE), residual toxicity, and environmental sustainability (Life Cycle Sustainability, LCS). The results indicated that H2O2 and ClO2, at relatively low concentrations, achieved significant microbial reduction while maintaining mycelial growth and yields comparable to those obtained with physical sterilization. However, further research is required to address the issues related to species-specific sensitivity and chemical residue safety. The development of biodegradable natural sterilizing agents and establishment of practical field guidelines are recommended to enhance the applicability of chemical sterilization methods in mushroom farming.
4,000원
3.
2025.09
구독 인증기관 무료, 개인회원 유료
The brix% of ASI 52822 resulted in the highest solid solubility. ASI 53399, ASI 50167, and ASI 52822 displayed high carbohydrate contents, whereas GBGA 01, GBGA 02, and ASI 52823 exhibited the half those values. The highest protein content was measured in ASI 52822. The antioxidant activity in the case of the hydrothermal method was lower than that for ascorbic acid, although it revealed more than 65% antioxidant activity. Furthermore, ASI 53399 and ASI 52822 exhibited antioxidant activities of 83.8% and 81.3%, respectively. To confirm the anticancer activity of the Ganoderma applanatum extract, we measured the original extract and also prepared a 10-time extract. The 10-time extract yielded strong anticancer activity, although the original extract did not exhibit any.
4,000원
4.
2025.09
구독 인증기관 무료, 개인회원 유료
Bone-related diseases (e.g., osteoporosis) represent a significant health challenge, prompting research for effective therapeutic agents, particularly from natural sources. The edible mushroom, Mycoleptodonoides aitchisonii has attracted interest due to its wide range of biological activities. Cytotoxicity assays revealed no significant toxicity of the M. aitchisonii water extract (MAWE) up to 50 μg/mL. MAWE significantly promoted dose-dependent osteoblast differentiation with ALP activity and mineralization increase by 109.17 % and 23 %, respectively, compared with the differentiation-only group. Moreover, MAWE significantly upregulated osteoblast-related gene expressions, including that of type I collagen (COL1A), osterix (Osx), and osteopontin (OPN). Furthermore, MAWE treatment significantly increased AMPK phosphorylation. This effect was further confirmed by demonstrating that the AMPK inhibitor compound C suppressed AMPK phosphorylation, and subsequent MAWE treatment restored it. In summary, these results demonstrate that MAWE possesses potent osteoblast differentiation-promoting efficacy, primarily through AMPK signaling pathway activation.
4,000원
5.
2025.09
구독 인증기관 무료, 개인회원 유료
In this study, we analyzed the contents of different bioactive compounds, including those with antioxidant activity, in selected mushroom species. Analysis of DPPH radical-scavenging activity revealed that Agaricus bisporus(‘A15’) had the highest antioxidant efficacy at 33.1 μg/mL, followed by Lentinula edodes and Pleurotus ostreatus, for which we also recorded high values. The results of nitrite-scavenging activity analysis revealed L. edodesto have the highest value at 15.8 μg/mL, with A. bisporus, P. ostreatus, and Wolfiporia extensaalso showing relatively good results. Furthermore, our analysis of total polyphenol contents indicated that A. bisporus had the highest content at 13.7 μg/mL, with L. edodesand Flammulina filiformis similarly having high levels, whereas W. extensa was established to contain the highest levels of β-glucan at 32.6 μg/mL, followed by L, edodes and P; ostreatus, which were also found to have significantly high values. These findings indicate that the antioxidant and nitrite-scavenging capacities and polyphenol and β-glucan contents differ according to fungal species. Among the evaluated species, given its high contents of a range of bioactive compounds, A. bisporus was assessed to be a suitable candidate for functional food development, Our findings in this study will provide essential basic data for the development of functional foods using edible mushroom species.
4,000원
6.
2025.09
구독 인증기관 무료, 개인회원 유료
Mycelium-based leather is a promising sustainable material that offers a biodegradable and animal-free alternative to conventional leather. However, ensuring its consistent quality during mass production remains a significant challenge owing to variability in biological growth and vulnerability to contamination. This study investigated the effects of surface modification and plasticization treatments on the structural and chemical properties of mycelial mats. Structural and chemical profile alterations were evaluated using hyperspectral imaging (HSI), Fourier-transform infrared spectroscopy, and scanning electron microscopy. HSI effectively detected these changes and surface defects nondestructively. It enabled precise differentiation between treated and untreated regions, thus supporting rapid and spatially resolved quality inspection. These results highlight the potential of HSI as an effective tool for evaluating the effects of pretreatment and monitoring contamination in mycelium-based leather production.
4,000원
7.
2025.09
구독 인증기관 무료, 개인회원 유료
A new oak mushroom cultivar Lentinula edodes ‘Eomjisong’ (LE23734), was developed by crossing the dikaryotic and monokaryotic strains of ‘KME36298’ and ‘KME36288-1’, respectively. The optimal temperature for mycelial growth of ‘Eomjisong’ on potato dextrose agar was 23–27°C, and was 15–20°C for fruiting body development. The cultivation period of ‘Eomjisong’ was 131 days, which was 3 days shorter than that of the control cultivar ‘Hanacham’. Morphologically, ‘Eomjisong’ showed stipe length and thickness similar to those of ‘Hanacham’, however, the pileus was larger and thicker. Furthermore, the pileus of ‘Eomjisong’ exhibited higher brightness than that of ‘Hanacham’. Productivity tests showed that the total yield of ‘Eomjisong’ reached 555 g, which was approximately 25.6% higher than that of ‘Hanacham’ (442 g). These findings indicate that ‘Eomjisong’ is a promising cultivar with enhanced productivity and morphological advantages over ‘Hanacham’, suggesting its potential for commercial cultivation.
4,000원
8.
2025.09
구독 인증기관 무료, 개인회원 유료
This study evaluated the effects of supplementing sawdust medium with 10% (w/w) Korean-style soy sauce residue (KS) and brewed soy sauce residue (BS) on the cultivation of Pleurotus ostreatus. Over 25 days, KS treatment produced mycelial growth comparable to the control, whereas BS treatment showed slower colonization. Fruiting bodies appeared after nine days in the control, ten days with BS, and twelve days with KS, with fresh weights of 146.0 g, 174.8 g, and 89.0 g, respectively, indicating reduced yield with KS and increased yield with BS. In antioxidant assays, DPPH radical scavenging slightly increased from 89.15% in the control to 89.99% with KS and 90.34% with BS. ABTS scavenging was 82.36% in the control, 83.42% with KS, and 75.99% with BS. Total polyphenol content was 64.39 mg AAE/mL in the control, 62.72 mg AAE/mL with KS, and 43.28 mg AAE/mL with BS. In conclusion, KS supports mycelial growth without compromising DPPH-based antioxidant capacity, while BS enhances fruiting body yield but reduces ABTS activity and phenolic content. Both residues show potential as supplementary nutrients in conventional sawdust media.
4,000원
9.
2025.09
구독 인증기관 무료, 개인회원 유료
This study aimed to select Perenniporia fraxinea isolates that form mycelial mats with excellent texture and ultimately to use them to produce mycelial based leather (MBL). Among 10 P. fraxinea isolates, JK-M0825 had the fastest mycelial growth rate on malt extract-yeast extract glucose medium. Polymorphic DNA of P. fraxinea isolates was detected using ultra-fast photonic polymerase chain reaction, which showed DNA diversity. The ribosomal DNA-internal transcribed spacer sequence of JK-M0825 was 99% identical to that of P. fraxinea isolates registered in GenBank. Four P. fraxinea isolates formed high-quality mycelial mats on sawdust substrates, and JK-M0825 produced the thickest mycelial mat (3.8 mm). JK-M0825 had the highest tensile strength (6.08 N/mm2) and an elongation rate of 32.93±6.31%. In addition, the mycelia of JK-M0825 formed uniformly on a large-scale culture medium (90 cm x 90 cm) and formed a textured mycelial mat. These results suggest that JK-M0825 is appropriate for producing MBL.
4,000원
10.
2025.09
구독 인증기관 무료, 개인회원 유료
This study reports the development of a new pure white Flammulina velutipes variety, ‘Seoldan’. The breeding process involved a primary cross between a brown strain (KMCC02267) collected by the Rural Development Administration and a white cultivar ‘Baekwoon’ (KMCC05472). From this cross, a white line ‘Fv-23-553’ was selected and subsequently crossed with a superior pure white resource (KMCC05474), resulting in the novel cultivar Seoldan. Mycelial growth tests showed that both varieties grew best at 25°C; however, Seoldan exhibited faster mycelial growth than the control variety under four temperature conditions except at 20°C. In media tests, Seoldan also demonstrated superior growth on three media types, with the exception of YM medium. In sawdust bottle cultivation, The time from scratching to the first fruiting was 2 days shorter in Seoldan than in the control variety, contributing to an overall reduction in cultivation time. The most distinct morphological trait of Seoldan is its pure white cap color. The average yield was 218.8 ± 10.6 g per bottle, comparable to that of the control. Morphologically, Seoldan produced smaller pileus and thinner stipes than the control. Furthermore, somatic incompatibility tests confirmed that Seoldan is genetically distinct from the control variety. These results indicate that Seoldan is a promising pure white variety with stable productivity, improved cultivation efficiency, and clear genetic differentiation from existing varieties.
4,000원
11.
2025.09
구독 인증기관 무료, 개인회원 유료
This study was conducted to evaluate horticultural and herbal crop by-products as alternative substrates for the cultivation of Pleurotus eryngii. Chemical analysis showed that Substrate 3 contained 42.2% total carbon and 2.6% total nitrogen, comparable to the control substrate (42.9% and 2.7%). After 35 days of incubation, mycelial growth in treatments ranged from 134.3 to 140.7 mm, which was similar to or greater than that of the control (135.5 mm). Fruiting body yield in Substrate 3 (173.4 g/1,100 mL) was about 14% higher than Control A (152.6 g) at 15 days after scratching, while Substrate 4 (202.9 g) produced yields comparable to Control B (209.6 g) at 17 days. These results demonstrate that red ginseng marc can entirely replace corn germ meal without compromising growth or yield, suggesting its strong potential as a sustainable substrate for P. eryngii. In addition, to enhance the utilization of by-products as substrate resources, it is essential to first establish stable and large-scale supply chains.
4,000원
12.
2025.09
구독 인증기관 무료, 개인회원 유료
CRISPR/Cas9 ribonucleoprotein (RNP)-mediated gene editing has recently been applied to Ganoderma lucidum as a promising tool for functional genomics and strain improvement. However, the multinuclear nature of this basidiomycete can result in genetic mosaicism, raising concerns about the long-term stability of edited strains. In this study, we report the occurrence of revertant phenotypes in CRISPR/Cas9-edited transformants of G. lucidum. Although the edited colonies initially exhibited the expected phenotype, repeated subculturing led to the reappearance of wild-type phenotypes. PCR and sequencing analyses revealed the coexistence of edited and non-edited nuclei, and the progressive loss of edited genotypes over successive generations. These findings demonstrate that multinuclearity is a key factor contributing to the instability of CRISPR/Cas9-based edits in G. lucidum. This brief report provides the first direct documentation of revertant occurrence in edited G. lucidum strains and underscores the need for rigorous selection strategies and novel approaches to secure stable homokaryotic transformants in mushroom genetic engineering.
3,000원
13.
2025.09
구독 인증기관 무료, 개인회원 유료
This study was conducted to evaluate the potential of using domestic agricultural by-products—pruned branches of apple and pear trees, soybean stems, and spent cocopeat substrate—as alternative substrates for the cultivation of Flammulina velutipes. Chemical analysis showed that Substrate 1 (apple branches replacing corncob) and Substrate 2 (pear branches replacing corncob) had total carbon (42.6%) and total nitrogen (1.5%) contents most comparable to those of the Control. Mycelial growth in all mixed substrates exceeded that of the control. Fruiting body yield was higher in Substrate 2 (273.4 g/1,100mL) and Substrate 1 (238.0 g) compared with the Control (231.5 g). Fruiting body quality in Substrates 1 and 2 was equivalent to that of the control. These results indicate that woody by-products such as apple and pear pruned branches can serve as effective replacements corncob in F. velutipes cultivation.
4,000원
