This study was conducted to evaluate horticultural and herbal crop by-products as alternative substrates for the cultivation of Pleurotus eryngii. Chemical analysis showed that Substrate 3 contained 42.2% total carbon and 2.6% total nitrogen, comparable to the control substrate (42.9% and 2.7%). After 35 days of incubation, mycelial growth in treatments ranged from 134.3 to 140.7 mm, which was similar to or greater than that of the control (135.5 mm). Fruiting body yield in Substrate 3 (173.4 g/1,100 mL) was about 14% higher than Control A (152.6 g) at 15 days after scratching, while Substrate 4 (202.9 g) produced yields comparable to Control B (209.6 g) at 17 days. These results demonstrate that red ginseng marc can entirely replace corn germ meal without compromising growth or yield, suggesting its strong potential as a sustainable substrate for P. eryngii. In addition, to enhance the utilization of by-products as substrate resources, it is essential to first establish stable and large-scale supply chains.

• 조성연(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Seong-Yeon Jo (Mushroom Research Division, NIHHS, RDA, Eumseong 27709, Korea) Corresponding author
• 박혜성(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Hye-Sung Park (Mushroom Research Division, NIHHS, RDA, Eumseong 27709, Korea)
• 안기홍(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Gi-Hong An (Mushroom Research Division, NIHHS, RDA, Eumseong 27709, Korea)
• 이강효(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Kang-Hyo Lee (Mushroom Research Division, NIHHS, RDA, Eumseong 27709, Korea)
• 한경숙(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Gyeong-Suk Han (Mushroom Research Division, NIHHS, RDA, Eumseong 27709, Korea)