This study was conducted to selection and investigate appropriate conditions for mass production of antagonistic microbes to control cobweb disease caused by Cladobotryum mycophilum. A grampositive bacterium was isolated from spent substrate of Agaricus bisporus and showed significant antagonistic activity against Cladobotryum mycophilum. The bacterium was identified as Bacillus altitudinis. based on the cultural, biochemical and physiological characteristics, and 16S rRNA sequence. The isolate is saprophytic, but not parasitic nor pathogenic to cultivated mushroom whereas it showed strong inhibitory effects against C. mycophilum cells in vitro. The control efficacy of B. altitudinis HC7 against cobweb disease of C. mycophilum was up to 78.2% on Agaricus bisporus. The suppressive bacterium may be useful for the development of biocontrol system. To define the appropriate conditions for the mass production of the Bacillus altitudinis HC7, we have investigated appropriate culture conditions and effects of various nutrient source on the bacterial growth. The appropriate initial pH and temperature were determined as pH 6.0 and 30oC, respectively. The appropriate concentration of medium elements for the growth of pathogen inhibitor bacterium(Bacillus altitudinis HC7) was determined as follows: 3.0% soluble startch, 10% soytone, 1.0% (NH4)2HPO4, 1.0 mmol KCl, and 0.5% L-asparagine.

This study aimed to address the increasing demand for technologies preventing mushroom fly damage. By monitoring the annual occurrence patterns of pests over several years and accumulating data, we conducted an analysis to evaluate the efficacy of preventive measures applied before the surge in mushroom fly infestation, typically observed in May. For preemptive control, physical measures involved installing air curtains at cultivation facility entrances and placing sticky traps and insect traps around entry points to block external entry and reduce internal insect density. Additionally, we applied an organic agricultural material, Dalmatian chrysanthemum extract, weekly alongside chemical control measures. To assess the reduction in mushroom fly populations, yellow sticky traps (15×25 cm) were placed at three locations within the mushroom cultivation facility, and the occurrence patterns before and after implementing preventive measures were compared. Compared to conventional practices, the application of preventive techniques resulted in a significant reduction, with a 60% decrease from 15 levels of mushroom flies/m² to 6 levels of mushroom flies/m² in May and a 40% decrease from 10 levels of mushroom flies/m² to 6 levels of mushroom flies/m² in June. While achieving over 50% efficacy during the peak mushroom fly season with preventive measures, we identified complementary actions such as blocking external sources (gaps in cultivation facility doors) and maintaining cleanliness around cultivation facilities (proper disposal of spent substrate) for further improvement. Comprehensive analysis and safety studies, including correlation analysis with contaminants and pathogens, are recommended to ensure the widespread adoption of mushroom fly preventive techniques for safe and stable mushroom production in the agricultural sector.

Calcifying Epithelial OdontogenicTumor(CEOT), also known as Pindborg tumor is a rare lesion comprising about 1% of all odontogenic tumors mostly rising in the posterior of the mandible. It generally occurs in patients between 30-60 years old. This paper describes a case of CEOT in a 17 year-old female affecting the right mandible. Histopathologic findings showed sheets of polygonal tumor cells and pools of amorphous, eosinophilic amyloids with calcifications. 1 year after resection of the tumor, no clinical and radiographic signs of recurrence were observed.

Background : This study provides Profitability Analysis information on Baeksoooh (Cynanchum wilfordii), One of the Crops excluded from the production cost survey. and it will contribute to increase the income of farmers. The purpose of this study was to survey and analyse the farm business performance of new economic crops cultivated in small area. Method and Results : In this study, we selected Baeksoooh, which is also called Baekhasuo, as a subject of investigation, based on the roots of C. wilfordii Hemsley. We visited 20 farmers who cultivated Baeksoooh in Jecheon, we surveyed the quantity, price of farmhouse receipts, cost of each branch, the status of agricultural machinery and facilities, and We analyzed income, operating expenses, and income per unit area. and also We set up a proper management model. It can be cultivated in all regions of Korea, but it is the main production area of Jecheon in Chungcheongbuk-do under the cool climate conditions. Most are being traded for medicinal uses. Looking at the profitability per 10a by Baeksooh's business performance, The result of this study are follows as. In the case of the top 20% farms, the gross quantity of the C. wilfordii is 332 ㎏ per 10a, the gross income was 11,418, managing cost was 2,259, income was 9,158 thousand won per 10a. In the average farms surveyed, the gross quantity of the C. wilfordii is 205 ㎏ per 10a, the gross income was 4,611, managing cost was 2,418, income was 2,193, production cost was 3,169 and net income was 1,445 thousand won per 10a. In the lower 20% farms cultivation, the gross quantity of the C. wilfordii is 46 ㎏ per 10a, the gross income was 89, managing cost was 1,848, income was – 1.759 thousand won per 10a. The professional farm management scale based on Baeksoooh's income of 20 million won is 43.4 a, it can produce 890 ㎏ with 1,176 hours per years, and the income is 9,522 thousand won Conclusion : Because the Baeksoooh being processed and circulated as health functional food have raised questions about their feasibility, Production and consumption declined sharply. but Consumption is expected to increase gradually and it is likely to grow, but prices are expected to fluctuate. Therefore, The bottom 20% farmers should benchmark the top 20% farms and cultivate the appropriate scale. and It is necessary to cultivate in consideration of supply and demand of manpower as well as proper size and cultivation.

Ethyl (4-(2,3-dihydro- 1H-indene-5-carboxyamido) benzoate)는 노화방지 효과가 있으나 난 용성 및 결정화 특성을 가지고 있어 제형화 및 2차 가공을 통해 입자내 활성성분을 담지시키는데 어려움이 있으 며, 3,5,7-trihydroxy-4’-methoxy-8-prenylflavone는 플라보노이드(flavonoid)류의 일종으로 미백기능 을 가지고 있으나 복합제형에 사용할 경우 제형의 착색을 일으키는 물질 중 하나이다. 본 연구에서는 전기방사법 을 이용하여 유용성/난용성 생리활성성분인 ethyl (4-(2,3-dihydro-1H-indene-5-carboxyamido) benzoate) 와 3,5,7-trihydroxy-4’-methoxy-8-prenylflavone 성분을 각각 poly (methyl methacrylate)와 polycaprolactone 고분자 입자 내에 담지 시키고 이를 화장품 제형에 적용했을 때 나타나는 효과에 대해서 기술 하였다. 입자내 활성성분을 담지시킨 고분자 입자를 제조하는 과정에는 고분자의 농도, 노즐의 구조, 그리고 고 분자와 생리활성 성분의 상용성이 중요한 역할을 하였다. 아울러 3,5,7-trihydroxy-4’-methoxy-8-prenylflavone가 캡슐화된 polycaprolactone 고분자 입자를 금속산화물이 포함된 화장품 제형과 혼합하였을 경우, 고분자 입자는 캡슐화 되지 않은 flavonoid가 제형에 포함되었을 경우 나타나는 착색현상을 방지하는 데 효과적 일 수 있다는 결과를 확인하였다.