This review assessed the feasibility and effectiveness of chemical sterilization methods as alternatives to conventional physical sterilization methods, with a specific focus on mushroom substrate preparation. Physical sterilization methods, such as autoclaving, provides excellent sterilization efficacy, but are resource-intensive and costly, limiting accessibility for small-scale mushroom growers. This review systematically examined recent research on chemical sterilization agents, notably hydrogen peroxide (H2O2) and chlorine dioxide (ClO2), focusing on sterilization efficacy, biological efficiency (BE), residual toxicity, and environmental sustainability (Life Cycle Sustainability, LCS). The results indicated that H2O2 and ClO2, at relatively low concentrations, achieved significant microbial reduction while maintaining mycelial growth and yields comparable to those obtained with physical sterilization. However, further research is required to address the issues related to species-specific sensitivity and chemical residue safety. The development of biodegradable natural sterilizing agents and establishment of practical field guidelines are recommended to enhance the applicability of chemical sterilization methods in mushroom farming.

Abstract
서 론
멸균의 중요성과 물리/화학적 멸균 방식
    화학제의 미생물 제거 수준 및 특성
    전과정평가(LCA) 관점에서 본 각 멸균법 비교의 필요성
화학 멸균 연구의 미래과제
적 요
결 론
감사의 글
REFERENCES

• 고기원(조선대학교 생명화학공학과) | Gi-Won Goh (Department of Biochemical Engineering, Chosun University, 60 Chosundae 5 gil, Donggu, Gwangju 61452, Republic of Korea)
• 신준우(조선대학교 생명화학공학과) | Jun-woo Shin (Department of Biochemical Engineering, Chosun University, 60 Chosundae 5 gil, Donggu, Gwangju 61452, Republic of Korea)
• 신현재(조선대학교 생명화학공학과) | Hyun-Jae Shin (Department of Biochemical Engineering, Chosun University, 60 Chosundae 5 gil, Donggu, Gwangju 61452, Republic of Korea) Corresponding author