This study investigates the effects of marine biofouling on the propulsion performance of a 2.99-ton HDPE (High-Density Polyethylene) coastal fishing vessel through full-scale sea trials. Four operating conditions, clean hull, fouled hull, partially cleaned hull, and completely cleaned hull, were tested to analyze changes in ship speed and engine revolutions. The maximum speed decreased from 32.2 kn in the clean condition to 18.7 kn under fouling, corresponding to a 42% reduction in performance, and recovered to 29.0 kn (about 90% of the original speed) after complete cleaning. Additionally, immersion tests of HDPE, FRP, and aluminum panels showed that all materials experienced severe fouling during spring and summer; however, HDPE surfaces exhibited lower adhesion strength and easier removal of organisms. These findings confirm that hull cleanliness has a significant influence on the propulsion efficiency of small vessels and highlight the necessity of proactive cleaning to maintain HDPE vessel performance. Future research will include comparative evaluations with FRP and aluminum vessels to assess the substitution potential of HDPE hulls and provide technical evidence for the wider adoption of eco-friendly fishing vessels.