This study was conducted to determine the effect of water temperature during the gonadal inactive season on the sex ratio of Tegillarca granosa, a sequential hermaphroditic bivalve. The sex ratio (F:M) of the group reared in wild was 1:1.3 (n=40:52), and the female ratio was 43.5%. In experimental groups that experienced different water temperature conditions (5.0, 7.0, 9.0 and 11.0℃) in an indoor aquarium during the gonadal inactive stage, the male ratio tended to increase as the water temperature increased. The correlation between water temperature and sex ratio was calculated as R2=0.7748. The results suggest that for sequential hermaphroditic bivalves, population sizes may decrease as the proportion of females decreases if water temperatures continue to rise due to climate warming.

Introduction
Materials and methods
    1. Experimental animals
    2. Rearing of experimental animals
    3. Sex ratio
    4. Histological analysis
    5. Statistical analysis
Results
    1. Water temperature and salinity in the studyarea
    2. Survival rate
    3. Sex ratio
Discussion
Declaration of Competing Interest
Acknowledgements
References

• Mi Ae Jeon(South Sea Fisheries Research Institute, National Institute of Fisheries Science, Yeosu 59780, Korea)
• Jung Sick Lee(Department of Aqualife Medicine, Chonnam National University, Yeosu 59626, Korea) Corresponding author
• Hyeon Jin Kim(Department of Aqualife Medicine, Chonnam National University, Yeosu 59626, Korea, Regional Leading Research Center, Chonnam National University, Yeosu 59626, Korea)
• Hyejin Kim(Genetics and Breeding Research Center, National Institute of Fisheries Science, Geoje 53334, Korea)
• Jung Jun Park(Aquaculture Reseach Division, National Institute of Fisheries Science, Busan 46083, Korea)
• Hyun Park(Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea)